Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
348,961 |
+G |
intergenic (+165/‑275) |
prpB → / → prpC |
2‑methylisocitrate lyase/2‑methylcitrate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 348,957 | 1 | . | G | 100.0%
| 12.0
/ NA
| 5 | intergenic (+161/‑279) | prpB/prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (5/0); total (5/0) |
TGCGCCGCTGACGTGACTTATCAGGCCTAC‑GGGGTGCACTGAACTGTAGGTCGGATAAGACG > W3110S.gb/348928‑348989
|
tGCGCCGCTGACGTGACTTATCAGGCCTACGGGGGTGCACTGAACTGTAGGTCGGATAAGACg > 1:1357735/1‑63 (MQ=255)
tGCGCCGCTGACGTGACTTATCAGGCCTACGGGGGTGCACTGAACTGTAGGTCGGATAAGACg > 1:1517262/1‑63 (MQ=255)
tGCGCCGCTGACGTGACTTATCAGGCCTACGGGGGTGCACTGAACTGTAGGTCGGATAAGACg > 1:1587929/1‑63 (MQ=255)
tGCGCCGCTGACGTGACTTATCAGGCCTACGGGGGTGCACTGAACTGTAGGTCGGATAAGACg > 1:2248766/1‑63 (MQ=255)
tGCGCCGCTGACGTGACTTATCAGGCCTACGGGGGTGCACTGAACTGTAGGTCGGATAAGACg > 1:2683416/1‑63 (MQ=255)
|
TGCGCCGCTGACGTGACTTATCAGGCCTAC‑GGGGTGCACTGAACTGTAGGTCGGATAAGACG > W3110S.gb/348928‑348989
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
