Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,077,129 |
C→A |
G726C (GGT→TGT) |
putA ← |
fused DNA‑binding transcriptional regulator, proline dehydrogenase, and pyrroline‑5‑carboxylate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,077,129 | 0 | C | A | 100.0%
| 26.6
/ NA
| 10 | G726C (GGT→TGT) | putA | fused DNA‑binding transcriptional regulator, proline dehydrogenase, and pyrroline‑5‑carboxylate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (10/0); total (10/0) |
TTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCC > W3110S.gb/1077092‑1077154
|
ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:1109091/1‑63 (MQ=255)
ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:1212735/1‑63 (MQ=255)
ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:1889547/1‑63 (MQ=255)
ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:2772835/1‑63 (MQ=255)
ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:2799386/1‑63 (MQ=255)
ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:2832066/1‑63 (MQ=255)
ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:2835436/1‑63 (MQ=255)
ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:3011165/1‑63 (MQ=255)
ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:53630/1‑63 (MQ=255)
ttACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTcc > 1:599662/1‑63 (MQ=255)
|
TTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCC > W3110S.gb/1077092‑1077154
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
