Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,150,273 |
A→G |
intergenic (+5/‑63) |
plsX → / → fabH |
fatty acid/phospholipid synthesis protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,150,273 | 0 | A | G | 100.0%
| 160.2
/ NA
| 44 | intergenic (+5/‑63) | plsX/fabH | fatty acid/phospholipid synthesis protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/44); total (0/44) |
TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTA > W3110S.gb/1150234‑1150334
|
tgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc < 1:2739557/65‑1 (MQ=255)
tgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc < 1:2773941/65‑1 (MQ=255)
tgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc < 1:1237891/65‑1 (MQ=255)
tgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc < 1:704360/65‑1 (MQ=255)
tgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc < 1:144513/65‑1 (MQ=255)
tgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc < 1:2703427/65‑1 (MQ=255)
tgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc < 1:3074978/65‑1 (MQ=255)
tgctgGACGGTCGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc < 1:808065/65‑1 (MQ=255)
gctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc < 1:1129102/64‑1 (MQ=255)
gctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGc < 1:1421682/64‑1 (MQ=255)
tGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGt < 1:1046310/65‑1 (MQ=255)
tGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGt < 1:2791247/65‑1 (MQ=255)
tGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGt < 1:2508815/65‑1 (MQ=255)
tGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGt < 1:1445366/65‑1 (MQ=255)
tGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACCGt < 1:395312/65‑1 (MQ=255)
ggCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGt < 1:2144571/64‑1 (MQ=255)
gAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGt < 1:835969/54‑1 (MQ=255)
tAGCAGGGCTCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg < 1:1840038/65‑1 (MQ=255)
tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg < 1:3035699/65‑1 (MQ=255)
tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg < 1:2911405/65‑1 (MQ=255)
tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg < 1:397552/65‑1 (MQ=255)
tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg < 1:2856646/65‑1 (MQ=255)
tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg < 1:2798707/65‑1 (MQ=255)
tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg < 1:476414/65‑1 (MQ=255)
tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg < 1:983772/65‑1 (MQ=255)
tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg < 1:108964/65‑1 (MQ=255)
tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg < 1:2561230/65‑1 (MQ=255)
tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg < 1:1254306/65‑1 (MQ=255)
tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg < 1:1962419/65‑1 (MQ=255)
tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg < 1:1952190/65‑1 (MQ=255)
tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTCACTGAg < 1:2703512/65‑1 (MQ=255)
aGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg < 1:1377042/64‑1 (MQ=255)
aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa < 1:3079383/65‑1 (MQ=255)
aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa < 1:850038/65‑1 (MQ=255)
aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa < 1:122099/65‑1 (MQ=255)
aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa < 1:1618799/65‑1 (MQ=255)
aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa < 1:1825346/65‑1 (MQ=255)
aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa < 1:1858395/65‑1 (MQ=255)
aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa < 1:319026/65‑1 (MQ=255)
aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa < 1:2998533/65‑1 (MQ=255)
aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa < 1:2246912/65‑1 (MQ=255)
aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa < 1:2851277/65‑1 (MQ=255)
aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa < 1:2673113/65‑1 (MQ=255)
aGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa < 1:2684619/65‑1 (MQ=255)
|
TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTA > W3110S.gb/1150234‑1150334
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
