Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,533,715 |
T→C |
G62G (GGT→GGC) |
ydcC → |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,533,715 | 0 | T | C | 100.0%
| 26.9
/ NA
| 10 | G62G (GGT→GGC) | ydcC | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (0/10); total (0/10) |
CCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGA > W3110S.gb/1533692‑1533757
|
cccGATTTTTTGAAGCAATATGGCGATTTTG‑AAATGGTATTCCTGTTCACGACACCATTGCCaga < 1:266621/65‑1 (MQ=11)
ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga < 1:1766393/65‑1 (MQ=255)
ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga < 1:1961311/65‑1 (MQ=255)
ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga < 1:2322907/65‑1 (MQ=255)
ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga < 1:2357421/65‑1 (MQ=255)
ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga < 1:2621878/65‑1 (MQ=255)
ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga < 1:500269/65‑1 (MQ=255)
ccGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga < 1:897491/65‑1 (MQ=255)
ccGATTTTTTGAAGCAATATGGCGATTTTCAAAATGGTATTCCTGTTCACGACACCATTGCCaga < 1:2136494/65‑1 (MQ=255)
tttttGAAGCAATATGGCTATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCaga < 1:1095595/60‑1 (MQ=11)
|
CCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGA > W3110S.gb/1533692‑1533757
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
