Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	354	41.7	3110465	93.1%	2895842	61.9

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,549,446	+C	coding (332/3048 nt)	fdnG →	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,549,443	1	.	C	75.0%	63.4 / 16.8	24	coding (329/3048 nt)	fdnG	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand): ref base . (0/6); new base C (18/0); total (18/6)
Fisher's exact test for biased strand distribution p-value = 7.43e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GTTTGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAGCG > W3110S.gb/1549404‑1549482

gTTTGCTGGATTACGTCAACAGTGAGAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCt                <  1:1116599/65‑1 (MQ=255)

gTTTGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCt                <  1:2426692/65‑1 (MQ=255)

gTTTGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCt                <  1:2447492/65‑1 (MQ=255)

gTTTGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCt                <  1:1840771/65‑1 (MQ=255)

gTTTGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCt                <  1:1224485/65‑1 (MQ=255)

 tttGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCt                <  1:1376149/64‑1 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:2598934/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:989369/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:832667/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:703736/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:684063/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:572060/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:473869/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:359382/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:3050421/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:1198711/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:2346669/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:2186600/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:2138646/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:1839903/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:1740318/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgcg  >  1:1704056/1‑65 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgc   >  1:1072978/1‑64 (MQ=255)

               tCAACAGTGAAAACCGTCTGCGCTACCCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAgc   >  1:68291/1‑64 (MQ=255)

GTTTGCTGGATTACGTCAACAGTGAAAACCGTCTGCGCTA‑CCCGGAATATCGTGCGCCAGGTTCTGACAAATGGCAGCG > W3110S.gb/1549404‑1549482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A