Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,873,346 |
C→T |
L416L (CTC→CTT) |
yeaI → |
predicted diguanylate cyclase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,873,346 | 0 | C | T | 100.0%
| 181.0
/ NA
| 55 | L416L (CTC→CTT) | yeaI | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/39); total (16/39) |
CGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGAC > W3110S.gb/1873303‑1873405
|
cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAgg > 1:1574537/1‑57 (MQ=255)
cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAAt > 1:2611695/1‑64 (MQ=255)
cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc > 1:1989990/1‑65 (MQ=255)
cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc > 1:1185846/1‑65 (MQ=255)
cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc > 1:2558286/1‑65 (MQ=255)
cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc > 1:2304232/1‑65 (MQ=255)
cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc > 1:414911/1‑65 (MQ=255)
cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc > 1:573619/1‑65 (MQ=255)
cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc > 1:954022/1‑65 (MQ=255)
cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc > 1:730080/1‑65 (MQ=255)
cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc > 1:663179/1‑65 (MQ=255)
cgcgACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACAGAACTTAATAGTGCCCAGGCAAAAATc > 1:2977255/1‑65 (MQ=255)
ggAGGTTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:1360671/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTgggg < 1:3757/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:373521/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:998172/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:2731221/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:774360/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:2806494/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:2828060/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:2927771/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:402397/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:2645112/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:465751/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:518117/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:570910/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:663922/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:58360/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:2536814/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:1549877/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:1721304/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:1842437/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:1872550/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:1936332/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:1973918/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:2011363/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:2107168/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:2280464/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:2272161/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:2405720/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:2409337/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:2471927/65‑1 (MQ=255)
ggAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:263976/65‑1 (MQ=255)
gAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCgg < 1:1915570/64‑1 (MQ=255)
gggTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGaa < 1:1241151/64‑1 (MQ=255)
gggTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGaa < 1:907916/64‑1 (MQ=255)
gggTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGaa < 1:837955/64‑1 (MQ=255)
gggTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGaa < 1:2827108/64‑1 (MQ=255)
gggTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGaa < 1:1769619/64‑1 (MQ=255)
gggTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGaa < 1:306028/64‑1 (MQ=255)
ggTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGaa < 1:835827/63‑1 (MQ=255)
ttGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATg > 1:995327/1‑54 (MQ=255)
ttGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATg > 1:2846667/1‑64 (MQ=255)
ttGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATg > 1:1270437/1‑64 (MQ=255)
gAACTTAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGAc > 1:1337922/1‑65 (MQ=255)
|
CGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGAC > W3110S.gb/1873303‑1873405
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
