Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,560,022 |
G→A |
P203S (CCT→TCT) |
yfeG ← |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,560,022 | 0 | G | A | 100.0%
| 138.4
/ NA
| 44 | P203S (CCT→TCT) | yfeG | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (33/11); total (33/11) |
TTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACA > W3110S.gb/2559986‑2560074
|
ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa > 1:901840/1‑65 (MQ=255)
ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa > 1:870115/1‑65 (MQ=255)
ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa > 1:1333970/1‑65 (MQ=255)
ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa > 1:1357888/1‑65 (MQ=255)
ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa > 1:3092359/1‑65 (MQ=255)
ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa > 1:2125462/1‑65 (MQ=255)
ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa > 1:2634910/1‑65 (MQ=255)
ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa > 1:2500336/1‑65 (MQ=255)
ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa > 1:1716435/1‑65 (MQ=255)
ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa > 1:1720025/1‑65 (MQ=255)
ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa > 1:1849574/1‑65 (MQ=255)
ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaa > 1:1827094/1‑65 (MQ=255)
ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCa > 1:2159714/1‑64 (MQ=255)
ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTAGCaa > 1:2371726/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGt > 1:469770/1‑64 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:816429/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:2549916/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:2485811/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:558520/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:517557/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:373068/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:477281/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:2174965/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:2055962/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:1764443/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:1695988/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:1662856/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:1582787/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:1499608/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:1483575/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:1288340/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTc > 1:1188740/1‑65 (MQ=255)
aaTTATCCCCCAGCACTTTTCGCACTCCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGt > 1:312797/1‑64 (MQ=255)
cGCACTGCAGACTGATTGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa < 1:351627/63‑1 (MQ=255)
cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa < 1:1135749/63‑1 (MQ=255)
cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa < 1:738077/63‑1 (MQ=255)
cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa < 1:925643/63‑1 (MQ=255)
cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa < 1:2901787/63‑1 (MQ=255)
cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa < 1:2751273/63‑1 (MQ=255)
cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa < 1:2683161/63‑1 (MQ=255)
cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa < 1:2520552/63‑1 (MQ=255)
cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa < 1:24494/63‑1 (MQ=255)
cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa < 1:2179917/63‑1 (MQ=255)
cGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa < 1:2073783/63‑1 (MQ=255)
|
TTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACA > W3110S.gb/2559986‑2560074
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
