Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,277,106 |
A→G |
T84A (ACT→GCT) |
sohA → |
predicted regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,277,106 | 0 | A | G | 100.0%
| 214.5
/ NA
| 58 | T84A (ACT→GCT) | sohA | predicted regulator |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/39); total (19/39) |
ATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACG > W3110S.gb/3277044‑3277157
|
aTCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCt < 1:363308/65‑1 (MQ=255)
aTCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCt < 1:1348030/65‑1 (MQ=255)
aTCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCt < 1:1038110/65‑1 (MQ=255)
tCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCt < 1:2414665/64‑1 (MQ=255)
aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc > 1:2426549/1‑65 (MQ=255)
tATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc > 1:806299/1‑63 (MQ=255)
tATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTcc > 1:2823042/1‑63 (MQ=255)
aTGAATTCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat < 1:3007190/64‑1 (MQ=255)
aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat < 1:2591527/64‑1 (MQ=255)
aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat < 1:988713/64‑1 (MQ=255)
aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat < 1:462127/64‑1 (MQ=255)
aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat < 1:321326/64‑1 (MQ=255)
aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat < 1:291761/64‑1 (MQ=255)
aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat < 1:2554491/64‑1 (MQ=255)
aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat < 1:1113502/64‑1 (MQ=255)
aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat < 1:1726835/64‑1 (MQ=255)
cATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCt < 1:1844291/50‑1 (MQ=255)
tttGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaacattca > 1:1934522/1‑63 (MQ=255)
tttGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaacattca > 1:60114/1‑63 (MQ=255)
tttGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaacattca > 1:499396/1‑63 (MQ=255)
tttGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaacattca > 1:405643/1‑63 (MQ=255)
ggATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcat < 1:2950116/41‑1 (MQ=255)
gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg < 1:2633305/64‑1 (MQ=255)
gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg < 1:2878088/64‑1 (MQ=255)
gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg < 1:1753502/64‑1 (MQ=255)
gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg < 1:2913401/64‑1 (MQ=255)
gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg < 1:1284179/64‑1 (MQ=255)
gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg < 1:1266223/64‑1 (MQ=255)
gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg < 1:1180761/64‑1 (MQ=255)
gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg < 1:611552/64‑1 (MQ=255)
gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg < 1:823858/64‑1 (MQ=255)
gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg < 1:860429/64‑1 (MQ=255)
gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg < 1:1698686/64‑1 (MQ=255)
gATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCg < 1:2404053/64‑1 (MQ=255)
atCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTgg < 1:1803796/65‑1 (MQ=255)
atCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTgg < 1:1842325/65‑1 (MQ=255)
atCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTgg < 1:1131991/65‑1 (MQ=255)
atCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTgg < 1:2464799/65‑1 (MQ=255)
atCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTgg < 1:570246/65‑1 (MQ=255)
acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt > 1:1205526/1‑50 (MQ=255)
acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt > 1:925834/1‑50 (MQ=255)
acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt > 1:2029491/1‑50 (MQ=255)
acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt > 1:2051970/1‑50 (MQ=255)
acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt > 1:59747/1‑50 (MQ=255)
acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt > 1:2077122/1‑50 (MQ=255)
acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt > 1:305377/1‑50 (MQ=255)
acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt > 1:1716899/1‑50 (MQ=255)
acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt > 1:2930589/1‑50 (MQ=255)
acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt > 1:2608815/1‑50 (MQ=255)
acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt > 1:2832265/1‑50 (MQ=255)
acaacCCGCAAAAAGCTCATCCATTCAACATTCAACAAGGAAAGAAACtt > 1:1459635/1‑50 (MQ=255)
caacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACg < 1:2103825/65‑1 (MQ=255)
caacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACg < 1:1223627/65‑1 (MQ=255)
caacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACg < 1:582788/65‑1 (MQ=255)
caacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACg < 1:1354807/65‑1 (MQ=255)
caacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACg < 1:2631492/65‑1 (MQ=255)
aacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACg < 1:1770329/64‑1 (MQ=255)
aacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACg < 1:2689567/64‑1 (MQ=255)
|
ATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACG > W3110S.gb/3277044‑3277157
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
