Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,473,895 |
A→G |
E162G (GAG→GGG) |
trmA → |
tRNA (uracil‑5‑)‑methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,473,895 | 0 | A | G | 100.0%
| 181.3
/ NA
| 50 | E162G (GAG→GGG) | trmA | tRNA (uracil‑5‑)‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (36/14); total (36/14) |
CTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGT > W3110S.gb/3473837‑3473956
|
cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg > 1:1122767/1‑64 (MQ=255)
cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg > 1:1974396/1‑64 (MQ=255)
cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg > 1:2530781/1‑64 (MQ=255)
cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg > 1:2063869/1‑64 (MQ=255)
cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg > 1:1664113/1‑64 (MQ=255)
cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg > 1:2092700/1‑64 (MQ=255)
cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg > 1:1597386/1‑64 (MQ=255)
cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg > 1:1549672/1‑64 (MQ=255)
cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTgg > 1:1266802/1‑64 (MQ=255)
cTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTg > 1:2123190/1‑63 (MQ=255)
gcgcgcgcAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATc > 1:1423222/1‑65 (MQ=255)
aaTGTGCATCTGGTTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:3053521/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGTTGGATCAGGATTACATCGATGa > 1:363097/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATg > 1:1252688/1‑64 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:2391918/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:2390411/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:241961/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:718301/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:2239056/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:2458310/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:255005/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:2995682/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:3031883/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:572154/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:665394/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:705831/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:1011081/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:1249363/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:141248/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:2164609/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:2004807/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:1518322/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:219585/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:1660711/1‑65 (MQ=255)
aaTGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGa > 1:1617989/1‑65 (MQ=255)
tGGTCGGGCAACGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGt > 1:490960/1‑63 (MQ=255)
aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga < 1:2291847/65‑1 (MQ=255)
aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga < 1:1800053/65‑1 (MQ=255)
aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga < 1:2482307/65‑1 (MQ=255)
aCGAAAACCAAAATCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAaga < 1:3029725/65‑1 (MQ=255)
tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt < 1:2729391/65‑1 (MQ=255)
tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt < 1:1469415/65‑1 (MQ=255)
tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt < 1:342106/65‑1 (MQ=255)
tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt < 1:173945/65‑1 (MQ=255)
tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt < 1:176315/65‑1 (MQ=255)
tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt < 1:1784902/65‑1 (MQ=255)
tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt < 1:2144492/65‑1 (MQ=255)
tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt < 1:2307317/65‑1 (MQ=255)
tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGt < 1:2298903/65‑1 (MQ=255)
tCGGGCTGGATCAGGATTACATCGATGAACGTCTGCCGGGCGCAGGGAAAGAGATGATCTACCGt < 1:1146201/65‑1 (MQ=255)
|
CTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGT > W3110S.gb/3473837‑3473956
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
