Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,582,634 |
A→G |
D411G (GAC→GGC) |
glnG → |
fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,582,634 | 0 | A | G | 100.0%
| 81.9
/ NA
| 23 | D411G (GAC→GGC) | glnG | fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (9/14); total (9/14) |
TCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGACAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAG > W3110S.gb/3582585‑3582680
|
tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc > 1:759888/1‑65 (MQ=255)
tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc > 1:1589719/1‑65 (MQ=255)
tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc > 1:1809065/1‑65 (MQ=255)
tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc > 1:1935549/1‑65 (MQ=255)
tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc > 1:2102280/1‑65 (MQ=255)
tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc > 1:684323/1‑65 (MQ=255)
tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc > 1:2173494/1‑65 (MQ=255)
tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc > 1:45766/1‑65 (MQ=255)
tCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGGCAGAGCGCTGCGTTc > 1:317029/1‑65 (MQ=255)
ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg < 1:2190299/65‑1 (MQ=255)
ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg < 1:999240/65‑1 (MQ=255)
ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg < 1:754898/65‑1 (MQ=255)
ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg < 1:51556/65‑1 (MQ=255)
ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg < 1:2685279/65‑1 (MQ=255)
ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg < 1:2656457/65‑1 (MQ=255)
ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg < 1:2563165/65‑1 (MQ=255)
ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg < 1:221129/65‑1 (MQ=255)
ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg < 1:1164889/65‑1 (MQ=255)
ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg < 1:218939/65‑1 (MQ=255)
ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg < 1:2165186/65‑1 (MQ=255)
ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg < 1:1448363/65‑1 (MQ=255)
ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg < 1:1395529/65‑1 (MQ=255)
ttcttGCGCAGTGGGCAGGCAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAg < 1:1295907/65‑1 (MQ=255)
|
TCGCAAATGCAACCGGACAGCTGGGCGACGCTTCTTGCGCAGTGGGCAGACAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAG > W3110S.gb/3582585‑3582680
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
