Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R2
|
278 |
43.2 |
2215644 |
94.6% |
2095999 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,291,126 |
G→A |
16.3% |
intergenic (+241/+212) |
yeeN → / ← asnW |
conserved hypothetical protein/tRNA‑Asn |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,291,126 | 0 | G | A | 16.3%
| 125.4
/ 14.9
| 49 | intergenic (+241/+212) | yeeN/asnW | conserved hypothetical protein/tRNA‑Asn |
| Reads supporting (aligned to +/- strand): ref base G (18/23); new base A (2/6); total (20/29) |
| Fisher's exact test for biased strand distribution p-value = 4.45e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 |
TACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGA > minE/1291059‑1291187
|
tACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTAc > 1:1090032/1‑71 (MQ=255)
aCGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTtata > 1:510611/1‑69 (MQ=255)
aCGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACg > 1:2060597/1‑71 (MQ=255)
tAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTAc < 1:21546/70‑1 (MQ=255)
tAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTAc > 1:32409/1‑70 (MQ=255)
gTTTCCCGATAAAGACGCTATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACtt > 1:1810901/1‑70 (MQ=255)
gTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACtt < 1:2041757/70‑1 (MQ=255)
gTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACtt > 1:1736001/1‑70 (MQ=255)
tttCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCa > 1:2212776/1‑71 (MQ=255)
aTAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACa < 1:1265837/71‑1 (MQ=255)
gATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTAc < 1:115715/45‑1 (MQ=255)
gATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTAc < 1:1865195/45‑1 (MQ=255)
gATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGt < 1:1019417/48‑1 (MQ=255)
tAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACgg > 1:1201942/1‑45 (MQ=255)
cGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTg < 1:509118/65‑1 (MQ=255)
aTCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACgg < 1:842077/38‑1 (MQ=255)
aTCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACgg < 1:1899646/38‑1 (MQ=255)
aTCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACgg < 1:2024211/38‑1 (MQ=255)
aTCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGaa < 1:743328/71‑1 (MQ=255)
aTCCCGCATAAGGAATTTTTCTCTTTATCTTTGTACgg < 1:1506422/38‑1 (MQ=255)
tCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCtt < 1:2071340/65‑1 (MQ=255)
tCCCGCATAAGGCATTTTTCTCTTTATCTTTATACGGTACTTCATGGaa < 1:454761/49‑1 (MQ=255)
ccGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGAcc > 1:1858906/1‑61 (MQ=255)
cGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt > 1:422522/1‑71 (MQ=255)
cGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:1870236/71‑1 (MQ=255)
cATAAGTCATTTTTCTCTTTATCTTTGTACGGTACTTCa < 1:347321/39‑1 (MQ=255)
cATAAGGCATTTTTCTCTTTATCTTTGTACGGTACtt < 1:1322790/37‑1 (MQ=255)
tAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGaa > 1:1308440/1‑42 (MQ=255)
aaGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGa > 1:1960951/1‑68 (MQ=255)
aGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTc < 1:931697/71‑1 (MQ=255)
gCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTg < 1:1951452/71‑1 (MQ=255)
cATTTTTCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGt > 1:506842/1‑71 (MQ=255)
tttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:1539108/60‑1 (MQ=255)
tttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGt < 1:2086261/69‑1 (MQ=255)
tttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTg < 1:840186/71‑1 (MQ=255)
tttttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGtt < 1:2075641/70‑1 (MQ=255)
ttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:365737/59‑1 (MQ=255)
ttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGt < 1:189591/59‑1 (MQ=255)
ttttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTg < 1:266453/70‑1 (MQ=255)
ttttCTCTTTATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTgg > 1:731967/1‑71 (MQ=255)
ttCTCTTTATCTTTGTACGTTACTTCATGGAACAGAGttt > 1:489183/1‑40 (MQ=255)
ttCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGa > 1:1364190/1‑59 (MQ=255)
tttATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGa > 1:512693/1‑39 (MQ=255)
tttATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGgggaggga > 1:1931186/1‑71 (MQ=255)
tttATCTTTATACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGgggagg < 1:1325900/69‑1 (MQ=255)
tATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTggg < 1:600280/63‑1 (MQ=255)
tATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTg > 1:730115/1‑61 (MQ=255)
tCTTTGTACGGTACTTCATGGAACAGAGTTTTTGCCCTTGCGAATCGTGATGTCTGTTGggga > 1:1933247/1‑63 (MQ=255)
tCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGggga > 1:533573/1‑63 (MQ=255)
tttGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGt < 1:159227/51‑1 (MQ=255)
|
TACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTCTTTATCTTTGTACGGTACTTCATGGAACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGA > minE/1291059‑1291187
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
