Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R2
|
278 |
43.2 |
2215644 |
94.6% |
2095999 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,467,238 |
G→T |
16.6% |
L234L (CTG→CTT) |
atpA → |
F1 sector of membrane‑bound ATP synthase, alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,467,238 | 0 | G | T | 16.6%
| 98.3
/ 5.3
| 36 | L234L (CTG→CTT) | atpA | F1 sector of membrane‑bound ATP synthase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (23/7); new base T (3/3); total (26/10) |
| Fisher's exact test for biased strand distribution p-value = 3.17e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 |
GGAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGA > minE/2467172‑2467308
|
ggAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCaa > 1:1270926/1‑70 (MQ=255)
gAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTTCACTTCAAt < 1:1761504/70‑1 (MQ=255)
gAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATa < 1:518883/71‑1 (MQ=255)
gcgcACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCg > 1:1111233/1‑71 (MQ=255)
gcgcACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCg > 1:1982997/1‑71 (MQ=255)
gcgcACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCg < 1:37255/71‑1 (MQ=255)
cacCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcg > 1:169852/1‑71 (MQ=255)
cacCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcg > 1:1167503/1‑71 (MQ=255)
cacCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcg > 1:1758674/1‑71 (MQ=255)
acCATCGTTGTGGTAGCAACCGCGTCTTAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcgc < 1:2142054/71‑1 (MQ=255)
ccATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCa < 1:1820151/71‑1 (MQ=255)
ccATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcgc < 1:1329941/70‑1 (MQ=255)
aTCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAAt > 1:193760/1‑71 (MQ=255)
aTCGTTGTGGTAGCAACCGCGTCAGAATCCGCTGCACTGCAATAc > 1:1113052/1‑45 (MQ=255)
tCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTg > 1:1242036/1‑64 (MQ=255)
tCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCCTATGCCGGTTGCGCAAtt > 1:1790039/1‑70 (MQ=255)
cGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATgg > 1:1010536/1‑71 (MQ=255)
gTTGTGGTAGCAACCGCGTCTGAATCCTCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATggg > 1:992936/1‑71 (MQ=255)
ttGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTa > 1:390201/1‑52 (MQ=255)
gtgGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCg > 1:1702667/1‑70 (MQ=255)
tgGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGaa < 1:13102/71‑1 (MQ=255)
gTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTg > 1:1079173/1‑39 (MQ=255)
aaCCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGtt > 1:297523/1‑50 (MQ=255)
aaCCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTcc > 1:1350908/1‑71 (MQ=255)
aaCCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTcc < 1:1875194/71‑1 (MQ=255)
ccGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCTGTTGCGCAATGGGCGAATACTTCCGt < 1:696996/71‑1 (MQ=255)
ccGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGt < 1:1969641/71‑1 (MQ=255)
gcgTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGa > 1:810450/1‑71 (MQ=255)
gcgTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGa > 1:64501/1‑71 (MQ=255)
cgTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGAc > 1:1699021/1‑71 (MQ=255)
tCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcg > 1:699777/1‑71 (MQ=255)
tGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcgcg > 1:1652749/1‑71 (MQ=255)
tGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcgcg > 1:1707727/1‑71 (MQ=255)
gAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcgcg > 1:2201594/1‑70 (MQ=255)
aaTCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGt > 1:1727806/1‑71 (MQ=255)
gCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGt > 1:14291/1‑57 (MQ=255)
ctgcactgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGAt < 1:1654620/71‑1 (MQ=255)
tgcactgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGaa < 1:170733/67‑1 (MQ=255)
tgcactgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGaa < 1:1243379/67‑1 (MQ=255)
actgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATgcgc > 1:159225/1‑71 (MQ=255)
ctgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCt > 1:1777966/1‑71 (MQ=255)
tgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGa < 1:164052/64‑1 (MQ=255)
tgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGa < 1:121828/64‑1 (MQ=255)
gcaATACCTGGCACCGTATGCCGTTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa > 1:2151513/1‑71 (MQ=255)
gcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa > 1:150039/1‑71 (MQ=255)
gcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa > 1:1400615/1‑71 (MQ=255)
gcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa > 1:1044182/1‑71 (MQ=255)
|
GGAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGA > minE/2467172‑2467308
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
