Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R2
|
278 |
43.2 |
2215644 |
94.6% |
2095999 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,528,498 |
C→T |
21.8% |
D83N (GAT→AAT) |
dfp ← |
fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase, FMN‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,528,498 | 0 | C | T | 21.8%
| 117.1
/ 27.0
| 55 | D83N (GAT→AAT) | dfp | fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase, FMN‑binding |
| Reads supporting (aligned to +/- strand): ref base C (26/17); new base T (7/5); total (33/22) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
CGCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCG > minE/2528436‑2528562
|
cGCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt > 1:399179/1‑71 (MQ=255)
gCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATc < 1:534146/62‑1 (MQ=255)
gCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCAttt < 1:1843881/71‑1 (MQ=255)
cATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTAc > 1:1654053/1‑71 (MQ=255)
ccGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTa > 1:297880/1‑66 (MQ=255)
ccGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCa > 1:1469731/1‑70 (MQ=255)
ccGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAg > 1:995663/1‑71 (MQ=255)
ccGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAg > 1:314257/1‑71 (MQ=255)
agcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCaa < 1:1667221/71‑1 (MQ=255)
gcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTc < 1:1978690/68‑1 (MQ=255)
caACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAata > 1:331886/1‑71 (MQ=255)
caACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAata > 1:920627/1‑71 (MQ=255)
caACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAata > 1:1213465/1‑71 (MQ=255)
caACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAat > 1:197468/1‑70 (MQ=255)
acacGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATg > 1:187874/1‑71 (MQ=255)
cacGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAttt < 1:1184573/55‑1 (MQ=255)
acGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCACATTTACCCAGCTCAata > 1:2194052/1‑67 (MQ=255)
cGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAATATGGcc < 1:585091/71‑1 (MQ=255)
aTCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTa < 1:558972/48‑1 (MQ=255)
tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCaa < 1:70315/57‑1 (MQ=255)
tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCa < 1:61884/43‑1 (MQ=255)
cAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCa > 1:957722/1‑50 (MQ=255)
cAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCa > 1:1903493/1‑65 (MQ=255)
cAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCg > 1:909106/1‑70 (MQ=255)
aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAata > 1:509682/1‑57 (MQ=255)
aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAata > 1:2214075/1‑57 (MQ=255)
aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATTGcc > 1:1899626/1‑62 (MQ=255)
aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGc > 1:616752/1‑71 (MQ=255)
aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGc > 1:1104866/1‑71 (MQ=255)
aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGc > 1:2199288/1‑71 (MQ=255)
aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGc > 1:1056121/1‑71 (MQ=255)
aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGc > 1:1597189/1‑71 (MQ=255)
aTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCtt < 1:2085266/71‑1 (MQ=255)
tCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGc > 1:1411687/1‑35 (MQ=255)
tCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGc > 1:847287/1‑35 (MQ=255)
tGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCa < 1:1626797/49‑1 (MQ=255)
tGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCa < 1:1593899/49‑1 (MQ=255)
ccGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTAccc > 1:689082/1‑41 (MQ=255)
ccGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCt < 1:428948/71‑1 (MQ=255)
gTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:732650/46‑1 (MQ=255)
gTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGc < 1:1776820/71‑1 (MQ=255)
gTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGc < 1:1187164/71‑1 (MQ=255)
tGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCt < 1:1875935/68‑1 (MQ=255)
tGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCt < 1:1873274/68‑1 (MQ=255)
tGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGcc > 1:114650/1‑71 (MQ=255)
cAGGGGCGAGAATCACTAAATCAGCCCATTTACACAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCggg < 1:671515/71‑1 (MQ=255)
ggggCGAGAATCACTAAATTAGCCCATTTACCCAGCTCaa < 1:1652953/40‑1 (MQ=255)
gggCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTc > 1:1393538/1‑70 (MQ=255)
ggCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCca < 1:2125876/71‑1 (MQ=255)
gCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAata > 1:162344/1‑39 (MQ=255)
gCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAata > 1:507750/1‑39 (MQ=255)
gCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCcag < 1:1601090/71‑1 (MQ=255)
gagaATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCcagca < 1:555005/71‑1 (MQ=255)
aCTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTc > 1:260737/1‑71 (MQ=255)
tAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCg > 1:277525/1‑70 (MQ=255)
tAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCg > 1:2103672/1‑70 (MQ=255)
|
CGCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCG > minE/2528436‑2528562
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
