Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I1 R2
|
146 |
74.7 |
3299319 |
84.8% |
2797822 |
66.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,352,427 |
T→C |
N101D (AAC→GAC) |
thiD ← |
bifunctional hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,352,427 | 0 | T | C | 100.0%
| 143.7
/ NA
| 40 | N101D (AAC→GAC) | thiD | bifunctional hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (2/38); total (2/38) |
AACCGCCGAAGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTTTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTCCGCCAG > minE/1352362‑1352487
|
aaCCGCCGAAGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGa < 1:277766/71‑1 (MQ=255)
aaCCGCCGAAGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGa < 1:2007355/71‑1 (MQ=255)
aaCCGCCGAAGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGa < 1:1600966/71‑1 (MQ=255)
aaGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:38104/71‑1 (MQ=255)
aaGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:3231118/71‑1 (MQ=255)
aaGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:3037675/71‑1 (MQ=255)
aaGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:154918/71‑1 (MQ=255)
aaGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:2884738/71‑1 (MQ=255)
aaGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:157138/71‑1 (MQ=255)
aaGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:853346/71‑1 (MQ=255)
aaGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:1677827/71‑1 (MQ=255)
aaGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:2620317/71‑1 (MQ=255)
aaGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:1681583/71‑1 (MQ=255)
aaGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:2395998/71‑1 (MQ=255)
aaGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:1712273/71‑1 (MQ=255)
aaGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:914043/71‑1 (MQ=255)
aGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:101450/70‑1 (MQ=255)
tGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:2241090/67‑1 (MQ=255)
cagcGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:1477014/61‑1 (MQ=255)
agcGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:2312550/60‑1 (MQ=255)
gtgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCa < 1:1333650/49‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:276749/71‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:791820/71‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:759664/71‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:1222604/71‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:3111058/71‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:3011189/71‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:1567522/71‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:1919200/71‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:1214026/71‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:2746160/71‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:1680197/71‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:2569365/71‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:2457281/71‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:1698834/71‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:1757667/71‑1 (MQ=255)
tgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:1041021/71‑1 (MQ=255)
aGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTcc < 1:1600317/66‑1 (MQ=255)
gTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTCCGCCAg > 1:2752969/1‑70 (MQ=255)
gTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTCCGCCAg > 1:2158193/1‑70 (MQ=255)
|
AACCGCCGAAGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTTTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTCCGCCAG > minE/1352362‑1352487
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
