Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I3 R2
|
122 |
64.1 |
3058059 |
97.6% |
2984665 |
63.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,352,427 |
T→C |
N101D (AAC→GAC) |
thiD ← |
bifunctional hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,352,427 | 0 | T | C | 100.0%
| 172.5
/ NA
| 50 | N101D (AAC→GAC) | thiD | bifunctional hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/25); total (25/25) |
AGCAACCGCCGAAGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTTTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTCCGCCAGCATACCG > minE/1352359‑1352494
|
aGCAACCGCCGAAGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGtctt > 1:2642700/1‑71 (MQ=255)
cAACCGCCGAAGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTg > 1:2088795/1‑70 (MQ=255)
aaCCGCCGAAGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGa < 1:2146560/71‑1 (MQ=255)
aCCGCCGAAGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGAt > 1:915319/1‑71 (MQ=255)
cgccgAAGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCt < 1:1622140/71‑1 (MQ=255)
cgccgAAGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCt < 1:2264467/71‑1 (MQ=255)
cgAAGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGa > 1:2593400/1‑70 (MQ=255)
aaGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa < 1:1719862/71‑1 (MQ=255)
aGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAAc < 1:978715/71‑1 (MQ=255)
ggTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACg < 1:546499/71‑1 (MQ=255)
ggTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACg < 1:2301160/71‑1 (MQ=255)
gTGAAAGCAGCGGGTCGCCGATTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACg < 1:608610/70‑1 (MQ=255)
tGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTgg < 1:2711479/58‑1 (MQ=255)
tGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTgg < 1:713594/58‑1 (MQ=255)
aaaGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGAt > 1:2206034/1‑58 (MQ=255)
aaGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGc > 1:1287515/1‑70 (MQ=255)
agcGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGAt > 1:568519/1‑53 (MQ=255)
gTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGtt < 1:1325007/68‑1 (MQ=255)
cgccgcTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTg > 1:635688/1‑69 (MQ=255)
cgccgcTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGc < 1:1422508/70‑1 (MQ=255)
gccgcTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTc < 1:2836119/66‑1 (MQ=255)
cgcTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTgg < 1:2370449/41‑1 (MQ=255)
gcTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATaa > 1:2866316/1‑49 (MQ=255)
gcTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCt > 1:2005480/1‑64 (MQ=255)
gcTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCt > 1:981706/1‑64 (MQ=255)
cTTTTTGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGc > 1:984149/1‑71 (MQ=255)
tttGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACg > 1:1522359/1‑47 (MQ=255)
ttGCCAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTc < 1:2220650/70‑1 (MQ=255)
cAGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGt < 1:11605/52‑1 (MQ=255)
aGCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTc > 1:2896642/1‑65 (MQ=255)
gCATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAata < 1:1374516/71‑1 (MQ=255)
cATAACGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAatat > 1:706159/1‑71 (MQ=255)
aTAACGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATc < 1:1084974/71‑1 (MQ=255)
aTAACGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATc > 1:738518/1‑71 (MQ=255)
aaCGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCgg > 1:1751412/1‑71 (MQ=255)
aaCGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCgg > 1:1134124/1‑71 (MQ=255)
aaCGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCgg > 1:875671/1‑71 (MQ=255)
aCGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCg > 1:291342/1‑55 (MQ=255)
cGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCAc > 1:221523/1‑52 (MQ=255)
cGGTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCAc > 1:250542/1‑52 (MQ=255)
ggTGTCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGttt < 1:3040928/71‑1 (MQ=255)
gtgtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAatat < 1:1438109/64‑1 (MQ=255)
gtCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTc > 1:1249111/1‑42 (MQ=255)
tCGAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTCCGc > 1:1411745/1‑71 (MQ=255)
gAGTACCACGTCTTGGATCTGATAACGTTGCAACCGtt > 1:2571168/1‑38 (MQ=255)
gAGTACCACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTCCGcc < 1:90317/70‑1 (MQ=255)
ccACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTCCGCCAGCAt < 1:1280467/70‑1 (MQ=255)
ccACGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTCCGCCAGCAt < 1:1081295/70‑1 (MQ=255)
cACGTCTTGGATCTGATAACGTTGCAACCGGTCTGCCACCGCTTCAACAata < 1:1077620/52‑1 (MQ=255)
cGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTCCGCCAGCATAcc > 1:148547/1‑70 (MQ=255)
cGTCTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTCCGCCAGCATACCg < 1:2917974/71‑1 (MQ=255)
|
AGCAACCGCCGAAGGTGAAAGCAGCGGGTCGCCGCTTTTTGCCAGCATAACGGTGTCGAGTACCACGTTTTGGATCTGATAACGTTGCAACCGTTCTGCCACCGCTTCAACAATATCGGTTTCCGCCAGCATACCG > minE/1352359‑1352494
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
