breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 52,517 T→C E757E (GAA→GAG imp ← exported protein required for envelope biosynthesis and integrity
RA 91,462 T→C S56P (TCT→CCT)  lpxC → UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase
RA 144,272 C→A G194V (GGC→GTC)  yadB ← glutamyl‑Q tRNA(Asp) synthetase
RA 195,616 C→T L71L (CTG→CTA yaeF ← predicted lipoprotein
RA 204,511 C→T intergenic (+111/‑73) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 204,556 A→G intergenic (+156/‑28) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 400,332 A→G F402L (TTT→CTT)  dacA ← D‑alanyl‑D‑alanine carboxypeptidase
RA 575,416 T→C N135S (AAT→AGT)  ybjX ← conserved hypothetical protein
RA 668,428 C→A T589K (ACG→AAG)  uup → fused predicted transporter subunits and ATP‑binding components of ABC superfamily
RA 692,900 T→C A144A (GCT→GCC hyaD → protein involved in processing of HyaA and HyaB proteins
RA 782,109 T→C Q167R (CAG→CGG)  hlyE ← hemolysin E
RA 798,949 C→T M214I (ATG→ATA treA ← periplasmic trehalase
RA 1,000,186 G→A G179D (GGT→GAT)  ydgD → predicted peptidase
RA 1,013,547 G→A R245C (CGC→TGC)  fumC ← fumarate hydratase (fumarase C), aerobic Class II
RA 1,036,505 G→A A632A (GCG→GCA rsxC → fused predicted 4Fe‑4S ferredoxin‑type protein
RA 1,081,858 T→C N104S (AAC→AGC)  ydhY ← predicted 4Fe‑4S ferridoxin‑type protein
RA 1,097,909 T→C intergenic (+32/‑397) ydiK → / → ydiL predicted inner membrane protein/conserved hypothetical protein
RA 1,099,149 A→G T130A (ACA→GCA)  ydiM → predicted transporter
RA 1,151,211 A→G V2V (GTA→GTG ydjQ → endonuclease of nucleotide excision repair
RA 1,196,798 (C)6→7 coding (354/1911 nt) yoaA ← conserved hypothetical protein with nucleoside triphosphate hydrolase domain
RA 1,231,014 C→T G581D (GGT→GAT)  ptrB ← protease II
RA 1,252,050 T→C K6K (AAA→AAG yebC ← conserved hypothetical protein
RA 1,253,716 C→T A241A (GCG→GCA aspS ← aspartyl‑tRNA synthetase
RA 1,269,468 (A)7→8 intergenic (‑18/‑282) uvrC ← / → yedP excinuclease UvrABC, endonuclease subunit/conserved hypothetical protein
RA 1,302,228 C→T V285M (GTG→ATG)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase
RA 1,325,522 T→G K71T (AAA→ACA)  wcaL ← predicted glycosyl transferase
RA 1,330,586 A→G Y334H (TAC→CAC)  cpsG ← phosphomannomutase
RA 1,352,427 T→C N101D (AAC→GAC)  thiD ← bifunctional hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase
RA 1,439,412 G→A A297V (GCT→GTT)  ompC ← outer membrane porin protein C
RA 1,509,579 G→A A42T (GCA→ACA)  ackA → acetate kinase A and propionate kinase 2
RA 1,511,849 T→C W373R (TGG→CGG)  pta → phosphate acetyltransferase
RA 1,523,658 C→T Q153* (CAA→TAA)  flk → predicted flagella assembly protein
RA 1,536,834 C→T M1I (ATG→ATA) † yfcR ← predicted fimbrial‑like adhesin protein
RA 1,591,415 G→T K203N (AAG→AAT yfeS → conserved hypothetical protein
RA 1,643,860 A→G R9R (CGT→CGC purC ← phosphoribosylaminoimidazole‑succinocarboxamide synthetase
RA 1,647,049 C→T Q102* (CAA→TAA)  bcp → thiol peroxidase, thioredoxin‑dependent
RA 1,664,529 G→A I231I (ATC→ATT guaA ← GMP synthetase
RA 1,680,754 T→C Y1407C (TAC→TGC)  yfhM ← conserved hypothetical protein
RA 1,695,295 G→A Q239* (CAG→TAG)  yfhQ ← predicted methyltransferase
RA 1,700,462 A→G L24P (CTA→CCA)  hcaT ← predicted 3‑phenylpropionic transporter
RA 1,777,713 T→C N105D (AAT→GAT)  trmD ← tRNA (guanine‑1‑)‑methyltransferase
RA 1,784,184 T→C I223T (ATA→ACA)  yfjB → NAD kinase
RA 1,827,842 Δ1 bp coding (1085/2562 nt) mutS → methyl‑directed mismatch repair protein
RA 1,833,531 C→T R92C (CGC→TGC)  ygbL → predicted class II aldolase
RA 1,867,615 G→A T429I (ACC→ATC)  ygcU ← predicted FAD containing dehydrogenase
RA 1,892,019 +GC intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein
RA 2,029,965 A→G F294L (TTC→CTC)  parE ← DNA topoisomerase IV, subunit B
RA 2,187,308 T→C P183P (CCA→CCG nanT ← sialic acid transporter
RA 2,279,749 Δ1 bp coding (633/1101 nt) trmA → tRNA (uracil‑5‑)‑methyltransferase
RA 2,364,103 A→G W456R (TGG→CGG)  trkH ← potassium transporter
RA 2,371,447 A→G F214L (TTT→CTT)  fre ← flavin reductase
RA 2,450,298 A→G W78R (TGG→CGG)  yifE ← conserved hypothetical protein
RA 2,473,718 C→T F331F (TTC→TTT glmS → L‑glutamine:D‑fructose‑6‑phosphate aminotransferase
RA 2,516,656 C→T A102T (GCT→ACT)  trmH ← tRNA (Guanosine‑2'‑O‑)‑methyltransferase
RA 2,605,272 A→G L50L (CTT→CTC uspA ← universal stress global response regulator
RA 2,632,970 A→G K421R (AAA→AGA)  livM → leucine/isoleucine/valine transporter subunit
RA 2,639,808 A→G T245A (ACG→GCG) 
*147Q (TAA→CAA) 
ugpQ →
yhhA ←
glycerophosphodiester phosphodiesterase, cytosolic
conserved hypothetical protein
RA 2,681,918 A→G R598R (CGT→CGC yhgF ← predicted transcriptional accessory protein
RA 2,703,672 G→A A355T (GCC→ACC)  aroB → 3‑dehydroquinate synthase
RA 2,726,174 G→A R303R (CGC→CGT yheS ← fused predicted transporter subunits and ATP‑binding components of ABC superfamily
RA 2,802,519 T→A D196V (GAT→GTT)  qor ← quinone oxidoreductase, NADPH‑dependent

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ minE 251995 252052 58 15 [12] [14] 15 malZ maltodextrin glucosidase
* * ÷ minE 444389 444427 39 17 [14] [14] 15 kdpE DNA‑binding response regulator in two‑component regulatory system with KdpD
* * ÷ minE 899406 899471 66 15 [14] [14] 16 pspD peripheral inner membrane phage‑shock protein
* * ÷ minE 2357786–2357951 2359167–2358006 56–1382 15 [14] [14] 17 rrfA–[rrlA] rrfA,[rrlA]