Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R2
|
422 |
46.8 |
3726540 |
94.7% |
3529033 |
61.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,185,915 |
A→G |
48.8% |
M36T (ATG→ACG) |
potB ← |
polyamine transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,185,915 | 0 | A | G | 48.8%
| ‑5.3
/ 59.7
| 41 | M36T (ATG→ACG) | potB | polyamine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (7/14); new base G (16/4); total (23/18) |
| Fisher's exact test for biased strand distribution p-value = 4.37e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 |
CAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGAAAGA > W3110S.gb/1185854‑1185984
|
cAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACaa < 1:2584153/71‑1 (MQ=255)
cAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACaa < 1:3180569/71‑1 (MQ=255)
cAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACaa < 1:3660795/71‑1 (MQ=255)
tAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCGTTTTGACAAAAc > 1:2081059/1‑71 (MQ=255)
aGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCGTTTTGACAAAACt > 1:1270946/1‑71 (MQ=255)
aGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCGTTTTGACAAAACt > 1:1553967/1‑71 (MQ=255)
aGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACt > 1:2243479/1‑71 (MQ=255)
aCTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGc < 1:2105542/71‑1 (MQ=255)
agagCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCgg > 1:1650340/1‑71 (MQ=255)
aTCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCaaaa < 1:2512613/71‑1 (MQ=255)
tCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCaaa > 1:2375983/1‑69 (MQ=255)
gACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGGGTCGTCGCGGGTCAAAAAGCTg < 1:1593717/68‑1 (MQ=255)
gACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTg < 1:401774/68‑1 (MQ=255)
cgcgTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGc > 1:2104195/1‑70 (MQ=255)
tAGTTATCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGat > 1:34202/1‑71 (MQ=255)
tAGTTATCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGa > 1:2704446/1‑70 (MQ=255)
tAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGat < 1:925892/71‑1 (MQ=255)
tAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGat < 1:2649060/71‑1 (MQ=255)
aGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGat < 1:1483042/70‑1 (MQ=255)
tATCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGatcat > 1:2316298/1‑70 (MQ=255)
tATCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGatcat > 1:3591728/1‑70 (MQ=255)
tATCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGatcat > 1:3306076/1‑70 (MQ=255)
aTCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGatcat > 1:3164238/1‑69 (MQ=255)
tCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCGGGTGCCAATGAtcatca < 1:183280/70‑1 (MQ=255)
gCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCATGTTg > 1:2140386/1‑71 (MQ=255)
tAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCaaaaa > 1:2378096/1‑43 (MQ=255)
tAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCaaaaa > 1:841027/1‑43 (MQ=255)
tAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCaaaaa > 1:3229746/1‑43 (MQ=255)
tAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCaaaaa > 1:281681/1‑43 (MQ=255)
tAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGat < 1:2864198/58‑1 (MQ=255)
tAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGat < 1:2779319/58‑1 (MQ=255)
aaGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGatca > 1:1152492/1‑58 (MQ=255)
aaGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGTCAATGATCATCAGGTTggg > 1:1220071/1‑68 (MQ=255)
aaGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTggg > 1:3357043/1‑68 (MQ=255)
aaGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTggg > 1:3593840/1‑68 (MQ=255)
aCCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGa < 1:3107109/69‑1 (MQ=255)
ccATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGa > 1:2583777/1‑68 (MQ=255)
cATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGAAAGa < 1:198862/71‑1 (MQ=255)
cATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGAAAGa < 1:3430059/71‑1 (MQ=255)
cATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGAAAGa < 1:347191/71‑1 (MQ=255)
cATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGAAAGa < 1:72756/71‑1 (MQ=255)
|
CAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTGGGCAGAAAGA > W3110S.gb/1185854‑1185984
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
