Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R1
|
560 |
56.4 |
4650532 |
94.9% |
4413354 |
59.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
500,029 |
G→A |
43.2% |
L227L (CTG→CTA) |
gsk → |
inosine/guanosine kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 500,029 | 0 | G | A | 43.2%
| 19.3
/ 52.7
| 44 | L227L (CTG→CTA) | gsk | inosine/guanosine kinase |
| Reads supporting (aligned to +/- strand): ref base G (12/13); new base A (11/8); total (23/21) |
| Fisher's exact test for biased strand distribution p-value = 5.57e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01 |
ATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAA > W3110S.gb/499961‑500083
|
aTGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTagg > 1:1847414/1‑71 (MQ=255)
ccGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCAc > 1:4205769/1‑71 (MQ=255)
aaGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTAGGCACCAAg > 1:3421249/1‑71 (MQ=255)
aGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTAGGCACCAAGt > 1:1915005/1‑71 (MQ=255)
aGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTAGGCACCAAGt > 1:662302/1‑71 (MQ=255)
gCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGtt > 1:3306631/1‑71 (MQ=255)
cAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTAGGCACCAAGttt < 1:1543379/71‑1 (MQ=255)
aCCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGt < 1:1294041/71‑1 (MQ=255)
ccATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGt > 1:1770859/1‑70 (MQ=255)
cATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCa > 1:2625983/1‑71 (MQ=255)
aaaGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTAGGCACCAAGTTTGTCATTGc > 1:2544480/1‑71 (MQ=255)
gCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGt > 1:1158178/1‑62 (MQ=255)
ccATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCgag < 1:2467207/71‑1 (MQ=255)
cATTGAGTACGCGAAGAAATATAACGTACCGATGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCgag < 1:3130225/70‑1 (MQ=255)
aTTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGaa < 1:1655689/71‑1 (MQ=255)
aTTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTAGGCACCAAGTTTGTc < 1:3757622/60‑1 (MQ=255)
ttGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGt < 1:3940329/58‑1 (MQ=255)
ttGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTga > 1:1868351/1‑63 (MQ=255)
gAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTAGGCACCAAGtt > 1:4307474/1‑53 (MQ=255)
gAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTAGGCACCAAGtt > 1:880033/1‑53 (MQ=255)
gAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTAGGCACCAAGTTTGTCATTGCCGAGAATcc > 1:3457647/1‑71 (MQ=255)
gAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTAGGCACCAAGTTTGTCATTGCCGAGAATcc > 1:770529/1‑71 (MQ=255)
gAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTAGGCACCAAGTTTGTCATTGCCGAGAATc > 1:2937227/1‑70 (MQ=255)
aGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCg < 1:3035446/71‑1 (MQ=255)
aGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTAGGCACCAAGTTTGTCATTGCCGAGAATCCg < 1:3920553/71‑1 (MQ=255)
cgcgAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGt > 1:385293/1‑51 (MQ=255)
cgcgAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAgt > 1:1983391/1‑71 (MQ=255)
cgcgAAGAAATATAACGTACCGGTGGTGCTGACGCTAGGCACCAAGTTTGt < 1:1301764/51‑1 (MQ=255)
cgcgAAGAAATATAACGTACCGGTGGTGCTGACGCTAGGCACCAAGTTTGTCATTGCCGAGAATCCGCAgt < 1:1673000/71‑1 (MQ=255)
cgcgAAGAAATATAACGTACCGGTGGTGCTGACGCTAGGCACCAAGTTTGTCATTGCCGAGAATCCGCAgt > 1:399335/1‑71 (MQ=255)
aagaaATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAg < 1:761518/42‑1 (MQ=255)
gaaATATTACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGt < 1:4647313/45‑1 (MQ=255)
aaaTATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGt < 1:1870439/44‑1 (MQ=255)
aaaTATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGtggtgg > 1:4064832/1‑69 (MQ=255)
atatAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGgcag > 1:2609259/1‑70 (MQ=255)
tataACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCgaga < 1:533157/52‑1 (MQ=255)
tataACGTACCGGTGGTGCTGACGCTAGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGgcagca < 1:2557692/71‑1 (MQ=255)
taACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCAtt < 1:3980464/43‑1 (MQ=255)
taACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAAt > 1:1292722/1‑71 (MQ=255)
aCGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAAtt < 1:1466358/70‑1 (MQ=255)
ccGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCa < 1:3720859/70‑1 (MQ=255)
ccGGTGGTGCTGACGCTAGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCa < 1:825396/70‑1 (MQ=255)
ggtggtGCTGACGCTAGGCACCAAGTTTGTCATTGCCGAGAATc > 1:1591045/1‑44 (MQ=255)
cTGACGCTAGGCACCAAGTTTGTCATTGCCGAGAATCCGCAg < 1:290411/42‑1 (MQ=255)
aCGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCaaa > 1:4306214/1‑60 (MQ=255)
|
ATGCCGGAAGCAACCATGAAAGCCATTGAGTACGCGAAGAAATATAACGTACCGGTGGTGCTGACGCTGGGCACCAAGTTTGTCATTGCCGAGAATCCGCAGTGGTGGCAGCAATTCCTCAAA > W3110S.gb/499961‑500083
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
