Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I2 R1	28	4.9	280516	80.6%	226095	64.0

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,798,695	A→G	Q239R (CAA→CGA)	proV →	glycine betaine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,798,695	0	A	G	85.7%	11.4 / ‑2.4	7	Q239R (CAA→CGA)	proV	glycine betaine transporter subunit
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (5/1); total (5/2)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGAT  >  minE/1798637‑1798756

tttATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCGAAATGGTGAAGt                                                   >  1:150805/1‑71 (MQ=255)

  tATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCGAAATGGTGAAGTgg                                                 >  1:247372/1‑71 (MQ=255)

                                     gCGACCGAATTGCCATTATGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCaataat              >  1:194759/1‑71 (MQ=255)

                                     gCGACCGAATTGCCATTATGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCaataat              >  1:87352/1‑71 (MQ=255)

                                               tGCCATTATGCGAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAAt     >  1:194811/1‑70 (MQ=255)

                                                gCCATTATGCGAAATGGTGAAGTGGTACAGGTCGGCacac                                  <  1:22747/40‑1 (MQ=255)

                                                 ccATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGAt  <  1:94053/71‑1 (MQ=255)

TTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATTGCCATTATGCAAAATGGTGAAGTGGTACAGGTCGGCACACCGGATGAAATTCTCAATAATCCGGCGAATGAT  >  minE/1798637‑1798756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A