Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R1
|
328 |
90.1 |
2317887 |
85.2% |
1974839 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
259,496:1 |
+T |
100% |
intergenic (+75/+102) |
yajD → / ← tsx |
conserved hypothetical protein/nucleoside channel, receptor of phage T6 and colicin K |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 259,496 | 1 | . | T | 100.0%
| 73.2
/ NA
| 23 | intergenic (+75/+102) | yajD/tsx | conserved hypothetical protein/nucleoside channel, receptor of phage T6 and colicin K |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (0/23); total (0/23) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGC‑TTTTGTTGGCCGGACAAAGCG > minE/259452‑259517
|
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:2109999/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:930421/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:886630/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:877062/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:836041/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:738441/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:650312/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:56018/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:45104/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:2274818/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:2185400/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:1049222/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:1972085/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:1971344/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:1757414/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:1578379/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:1571131/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:154969/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:1499507/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:1321411/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:1203671/67‑1 (MQ=255)
cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:1071293/67‑1 (MQ=255)
ttATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTTGTTGGCCGGACAAAGCg < 1:806084/66‑1 (MQ=255)
|
CTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGC‑TTTTGTTGGCCGGACAAAGCG > minE/259452‑259517
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
