Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R1
|
328 |
90.1 |
2317887 |
85.2% |
1974839 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,205,451 |
A→T |
100% |
V17V (GTT→GTA) |
yoaE ← |
fused predicted membrane proteins |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,205,451 | 0 | A | T | 80.4%
| 101.0
/ 20.9
| 46 | V17V (GTT→GTA) | yoaE | fused predicted membrane proteins |
| Reads supporting (aligned to +/- strand): ref base A (9/0); new base T (0/37); total (9/37) |
| Fisher's exact test for biased strand distribution p-value = 9.08e-10 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
ATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTT > minE/1205428‑1205516
|
aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:219501/1‑67 (MQ=255)
aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:775442/1‑67 (MQ=255)
aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:2221956/1‑67 (MQ=255)
aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:31019/1‑67 (MQ=255)
aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:1529188/1‑67 (MQ=255)
aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:681498/1‑67 (MQ=255)
aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:637637/1‑67 (MQ=255)
aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:1502618/1‑67 (MQ=255)
aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCACGCCCAAATTGAGGGGTCCATTAAg > 1:385694/1‑66 (MQ=255)
acaTTTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1066259/67‑1 (MQ=255)
acaATTTCTAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:2007807/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGGCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:900431/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGTATTCCATGa < 1:576614/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCTATGa < 1:3929/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:104414/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:478081/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:370438/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:712942/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:315158/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:725613/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:308879/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:2304014/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:2271109/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:732834/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:812069/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:979969/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1395534/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1036292/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1062389/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:110663/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1106745/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1112338/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1215492/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1292487/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:13922/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:2018641/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1481581/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1532020/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1625434/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1745849/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1765021/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1799807/67‑1 (MQ=255)
acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1829967/67‑1 (MQ=255)
cattttCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1298449/63‑1 (MQ=255)
caATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1503797/66‑1 (MQ=255)
caATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:2054415/66‑1 (MQ=255)
caacaaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACaa < 1:1189132/57‑1 (MQ=255)
caacaaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACaa < 1:201583/57‑1 (MQ=255)
caacaaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACaa < 1:898731/57‑1 (MQ=255)
caacaaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACaa < 1:1051645/57‑1 (MQ=255)
caacaaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCtt < 1:2115140/67‑1 (MQ=255)
|
ATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTT > minE/1205428‑1205516
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
