Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R1
|
328 |
90.1 |
2317887 |
85.2% |
1974839 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,504,431 |
C→A |
100% |
S295R (AGC→AGA) |
yfbQ → |
predicted aminotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,504,431 | 0 | C | A | 100.0%
| 14.3
/ NA
| 6 | S295R (AGC→AGA) | yfbQ | predicted aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (6/0); total (6/0) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CGCTAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAA > minE/1504412‑1504478
|
cgcTAGGTGGTTATCAGAGAATCAGTGAATTTATTAcccc > 1:1142608/1‑40 (MQ=255)
cgcTAGGTGGTTATCAGAGAATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTaa > 1:1835250/1‑67 (MQ=255)
cgcTAGGTGGTTATCAGAGAATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTaa > 1:393958/1‑67 (MQ=255)
cgcTAGGTGGTTATCAGAGAATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTaa > 1:913765/1‑67 (MQ=255)
cgcTAGGTGGTTATCAGAGAATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTa > 1:830035/1‑66 (MQ=255)
cgcTAGGTGGTTATCAGAGAATCAGTGAATTTATTACCACTGGCGGTCGTCTTTATGAGCAGCGTaa > 1:1067451/1‑67 (MQ=255)
|
CGCTAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAA > minE/1504412‑1504478
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
