Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,021,506 |
C→T |
T512I (ACT→ATT) |
xdhD → |
fused predicted xanthine/hypoxanthine oxidase, molybdopterin‑binding subunit and Fe‑S binding subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,021,506 | 0 | C | T | 97.1%
| 106.4
/ ‑3.8
| 36 | T512I (ACT→ATT) | xdhD | fused predicted xanthine/hypoxanthine oxidase, molybdopterin‑binding subunit and Fe‑S binding subunit |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (17/17); total (19/17) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAGCGAAAAAAGATGGTCGCCTGACGGCAGTGAAGATGGATTTCCGCGCCAACACTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTCGC > W3110S.gb/3021452‑3021562
|
gAGCGAAAAAAGATGGTCGCCTGACGGCAGTGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAAc < 1:3531863/71‑1 (MQ=255)
gAGCGAAAAAAGATGGTCGCCTGACGGCAGTGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAAc < 1:1051290/71‑1 (MQ=255)
gAGCGAAAAAAGATGGTCGCCTGACGGCAGTGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAAc < 1:3126252/71‑1 (MQ=255)
gAGCGAAAAAAGATGGTCGCCTGACGGCAGTGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAAc < 1:2937935/71‑1 (MQ=255)
gAGCGAAAAAAGATGGTCGCCTGACGGCAGTGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAAc < 1:497581/71‑1 (MQ=255)
tGAAGATGGATTTCCGCGCCATCATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACgg < 1:3934650/67‑1 (MQ=255)
tGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACgg < 1:2223965/67‑1 (MQ=255)
tGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACgg < 1:3441650/67‑1 (MQ=255)
tGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACgg < 1:3584532/67‑1 (MQ=255)
tGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACgg < 1:3865427/67‑1 (MQ=255)
tGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACgg < 1:2539934/67‑1 (MQ=255)
tGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACgg < 1:1813491/67‑1 (MQ=255)
tGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACgg < 1:759203/67‑1 (MQ=255)
tGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACgg < 1:1573693/67‑1 (MQ=255)
tGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACgg < 1:1558154/67‑1 (MQ=255)
tGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACgg < 1:1384214/67‑1 (MQ=255)
tGAAGATGGATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACgg < 1:1226701/67‑1 (MQ=255)
gAAGATGGATTTCCGCGCCAACACTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCgg > 1:1618014/1‑71 (MQ=255)
ggATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTg > 1:3727505/1‑70 (MQ=255)
ggATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTg > 1:3384003/1‑70 (MQ=255)
ggATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGt > 1:3898209/1‑71 (MQ=255)
ggATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGt > 1:3555070/1‑71 (MQ=255)
ggATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGt > 1:3485978/1‑71 (MQ=255)
ggATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGt > 1:3490662/1‑71 (MQ=255)
gATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGTACCGGCGCTGTc > 1:801373/1‑71 (MQ=255)
gATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTc > 1:325972/1‑71 (MQ=255)
gATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTc > 1:3087369/1‑71 (MQ=255)
gATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTc > 1:2033823/1‑71 (MQ=255)
gATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTc > 1:68537/1‑71 (MQ=255)
gATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTc > 1:1726844/1‑71 (MQ=255)
gATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTc > 1:1521770/1‑71 (MQ=255)
gATTTCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGATGTc > 1:2059728/1‑71 (MQ=255)
gATTTCCGCGCCAACAATGGCCCTTACGGCAACCACTCACACACCGTACCGTGTAACg > 1:3209321/1‑58 (MQ=255)
tttCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTCg > 1:2572631/1‑70 (MQ=255)
tttCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTCGc > 1:2108961/1‑71 (MQ=255)
tttCCGCGCCAACATTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTCGc > 1:656381/1‑71 (MQ=255)
|
GAGCGAAAAAAGATGGTCGCCTGACGGCAGTGAAGATGGATTTCCGCGCCAACACTGGCCCTTACGGCAACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTCGC > W3110S.gb/3021452‑3021562
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
