Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R2
|
44 |
10.9 |
588504 |
78.3% |
460798 |
57.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,669,352:1 |
+AAC |
100% |
coding (691/1473 nt) |
der ← |
predicted GTP‑binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,669,352 | 1 | . | A | 100.0%
| 17.5
/ NA
| 7 | Y231? (TAC→NAC) | der | predicted GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/7); total (0/7) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
| * | minE | 1,669,352 | 2 | . | A | 100.0%
| 17.5
/ NA
| 7 | Y231? (TAC→NAC) | der | predicted GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/7); total (0/7) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
| * | minE | 1,669,352 | 3 | . | C | 100.0%
| 19.6
/ NA
| 7 | Y231D (TAC→GAC) | der | predicted GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (0/7); total (0/7) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TGTCACGCGTCGTGCCAGGCATGTCGTA‑‑‑AACAACAACGCGCTCTTCACCAAGAATACG > minE/1669325‑1669382
|||
tGTCACGCGTCGTGCCAGGCATGTCGTAAACAACAACAACGCGCTCTTCACCAAGAATACg < 1:150417/61‑1 (MQ=255)
aCGCGTCGTGCCAGGCATGTCGTAAACAACAACAACGCGCTCTTCACCAAGAATACg < 1:101962/57‑1 (MQ=255)
aCGCGTCGTGCCAGGCATGTCGTAAACAACAACAACGCGCTCTTCACCAAGAATACg < 1:44479/57‑1 (MQ=255)
aCGCGTCGTGCCAGGCATGTCGTAAACAACAACAACGCGCTCTTCACCAAGAATACg < 1:515247/57‑1 (MQ=255)
aCGCGTCGTGCCAGGCATGTCGTAAACAACAACAACGCGCTCTTCACCAAGAATACg < 1:555968/57‑1 (MQ=255)
aCGCGTCGTGCCAGGCATGTCGTAAACAACAACAACGCGCTCTTCACCAAGAATACg < 1:574444/57‑1 (MQ=255)
aCGCGTCGTGCCAGGCATGTCATAAACAACAACAACGCGCTCTTCACCAAGAATACg < 1:28112/57‑1 (MQ=255)
|||
TGTCACGCGTCGTGCCAGGCATGTCGTA‑‑‑AACAACAACGCGCTCTTCACCAAGAATACG > minE/1669325‑1669382
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
