Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R2
|
44 |
10.9 |
588504 |
78.3% |
460798 |
57.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,043,667 |
A→T |
100% |
R31W (AGG→TGG) |
yqiK → |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,043,667 | 0 | A | T | 100.0%
| 26.1
/ NA
| 10 | R31W (AGG→TGG) | yqiK | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (0/10); total (0/10) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAG > minE/2043631‑2043693
|
gTATGCATTCTGTTTATTA‑TAGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:231338/62‑1 (MQ=255)
tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:147906/62‑1 (MQ=255)
tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:334893/62‑1 (MQ=255)
tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:379084/62‑1 (MQ=255)
tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:396867/62‑1 (MQ=255)
tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:467367/62‑1 (MQ=255)
tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:48336/62‑1 (MQ=255)
tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:537020/62‑1 (MQ=255)
tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:64218/62‑1 (MQ=255)
tATGCATTCTGTTTATTATTGGAATTATCTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:574052/62‑1 (MQ=255)
|
GTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAG > minE/2043631‑2043693
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
