Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R2 230 93.6 4701257 96.3% 4527310 61.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 497,847 A→G 61.1% Y222H (TAT→CAT)  galE ← UDP‑galactose‑4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE497,8470AG61.1% 106.1 / 139.6 131Y222H (TAT→CAT) galEUDP‑galactose‑4‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (24/27);  new base G (26/54);  total (50/81)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01

Only 100 of 136 total aligned reads displayed.

ACGACGTGACCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATCAGGT  >  minE/497777‑497915
                                                                      |                                                                    
acgacgTTACCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATa                                                                      <  1:1731217/71‑1 (MQ=255)
acgacgTGACCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATa                                                                      <  1:4469401/71‑1 (MQ=255)
  gacgTGACCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGgatgat                                                                    >  1:3002866/1‑71 (MQ=255)
      tGACCGTCCGCCTGATCCATTTCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGtt                                                                <  1:4537535/71‑1 (MQ=255)
        aCCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTAc                                                              >  1:1002372/1‑71 (MQ=255)
        aCCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATAGTTAc                                                              >  1:2207481/1‑71 (MQ=255)
        aCCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTAc                                                              >  1:3295625/1‑71 (MQ=255)
         ccGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTAcc                                                             <  1:773555/71‑1 (MQ=255)
          cGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCg                                                                  <  1:2347635/65‑1 (MQ=255)
            tCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTTGGATGATCGTTACCaaa                                                          <  1:1751842/71‑1 (MQ=255)
            tCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCaaa                                                          <  1:1634269/71‑1 (MQ=255)
              cGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCaaaa                                                         <  1:4645788/70‑1 (MQ=255)
              cGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCaaaa                                                         <  1:2915562/70‑1 (MQ=255)
                 cAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCg                                                     >  1:4193978/1‑71 (MQ=255)
                   gATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCaaaa                                                         <  1:944947/65‑1 (MQ=255)
                      ccATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCTGATGATCGTTACCAAAAATCGCCAGc                                                >  1:2538671/1‑71 (MQ=255)
                         ttACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAg                                             <  1:1665154/71‑1 (MQ=255)
                         ttACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAg                                             <  1:3195512/71‑1 (MQ=255)
                         ttACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAg                                             <  1:4505300/71‑1 (MQ=255)
                         atACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAg                                             <  1:211748/70‑1 (MQ=255)
                           aCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTc                                           >  1:3869486/1‑71 (MQ=255)
                           aCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTc                                           >  1:1435776/1‑71 (MQ=255)
                           aCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTc                                           >  1:3875004/1‑71 (MQ=255)
                             gTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTcgc                                         <  1:668230/71‑1 (MQ=255)
                             gTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTcgc                                         >  1:3918842/1‑71 (MQ=255)
                             gTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTcgc                                         >  1:1567558/1‑71 (MQ=255)
                             gTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTcgc                                         <  1:130795/71‑1 (MQ=255)
                                 aTGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACg                                     <  1:2120846/71‑1 (MQ=255)
                                  tGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTcgc                                         <  1:1991597/66‑1 (MQ=255)
                                  tGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACgg                                    <  1:3729872/71‑1 (MQ=255)
                                  tGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACgg                                    <  1:3089/71‑1 (MQ=255)
                                    tAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGc                                                <  1:2148834/57‑1 (MQ=255)
                                    tAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGc                                   >  1:3014983/1‑70 (MQ=255)
                                     aaTCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGt                                                                 >  1:1966403/1‑39 (MQ=255)
                                     aaTCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGc                                   <  1:1301696/69‑1 (MQ=255)
                                     aaTCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGcc                                  >  1:613053/1‑70 (MQ=255)
                                     aaTCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGcc                                  >  1:377927/1‑70 (MQ=255)
                                      aTCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCt                                 <  1:425898/70‑1 (MQ=255)
                                      aTCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTa                                >  1:17805/1‑71 (MQ=255)
                                      aTCGCGTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTa                                <  1:1125128/71‑1 (MQ=255)
                                         gcgTACGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACa                              <  1:3563568/70‑1 (MQ=255)
                                         gcgTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAg                             >  1:4229149/1‑71 (MQ=255)
                                         gcgTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAg                             >  1:1750080/1‑71 (MQ=255)
                                           gTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCaaaa                                                         <  1:3880190/41‑1 (MQ=255)
                                           gTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCaaaa                                                         <  1:4002483/41‑1 (MQ=255)
                                            tACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATc                                                      <  1:3551000/43‑1 (MQ=255)
                                              cGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGc                                                <  1:1077121/47‑1 (MQ=255)
                                              cGCCAGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAAcc                        <  1:3915947/71‑1 (MQ=255)
                                              cGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAAcc                        >  1:1129861/1‑71 (MQ=255)
                                               gCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCa                                                  >  1:3572305/1‑44 (MQ=255)
                                                  aGTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTggg                    >  1:858424/1‑71 (MQ=255)
                                                   gTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTc                                           >  1:2471645/1‑47 (MQ=255)
                                                   gTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTc                                           >  1:275355/1‑47 (MQ=255)
                                                   gTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGcc                                  <  1:2308493/56‑1 (MQ=255)
                                                   gTACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGc                   <  1:4098780/71‑1 (MQ=255)
                                                    tACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGcc                                  <  1:3902485/55‑1 (MQ=255)
                                                    tACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAAc                         <  1:4224267/64‑1 (MQ=255)
                                                    tACCATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAAc                         <  1:4473442/64‑1 (MQ=255)
                                                    tACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGa                                              <  1:586624/43‑1 (MQ=255)
                                                    tACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACg                                     >  1:1134168/1‑52 (MQ=255)
                                                    tACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCaa                          <  1:3443943/63‑1 (MQ=255)
                                                    tACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCaa                          <  1:3506034/63‑1 (MQ=255)
                                                    tACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCaa                          <  1:370102/63‑1 (MQ=255)
                                                    tACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAAc                         <  1:87738/64‑1 (MQ=255)
                                                    tACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCg                  <  1:2261958/71‑1 (MQ=255)
                                                     aCCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACa                              >  1:2450768/1‑58 (MQ=255)
                                                      ccATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGa                 <  1:5799/70‑1 (MQ=255)
                                                      ccATCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGAt                >  1:3288982/1‑71 (MQ=255)
                                                      ccATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCATACAGCAACCTGGGCGAt                >  1:2337972/1‑71 (MQ=255)
                                                       cATCTTCGGTCGTATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg               <  1:2644012/71‑1 (MQ=255)
                                                       cATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg               <  1:3450098/71‑1 (MQ=255)
                                                       cATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg               <  1:1590987/71‑1 (MQ=255)
                                                       cATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg               <  1:2290848/71‑1 (MQ=255)
                                                       cATCTTCGGACGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg               <  1:4541826/71‑1 (MQ=255)
                                                        aTCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTTGGCGATGt              <  1:188391/71‑1 (MQ=255)
                                                         tCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCt                                 <  1:2556038/51‑1 (MQ=255)
                                                         tCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCaa                          <  1:1039840/58‑1 (MQ=255)
                                                         tCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCaa                          <  1:4678286/58‑1 (MQ=255)
                                                         tCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTa             >  1:1879790/1‑71 (MQ=255)
                                                         tCTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTa             >  1:707852/1‑71 (MQ=255)
                                                         tCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTa                                <  1:262141/52‑1 (MQ=255)
                                                         tCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg               <  1:191344/69‑1 (MQ=255)
                                                         tCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGAAACCTGGGCGATGTa             >  1:737783/1‑71 (MQ=255)
                                                          cTTCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTcgc                                         <  1:4543674/42‑1 (MQ=255)
                                                           ttCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGa                 >  1:3109557/1‑65 (MQ=255)
                                                           ttCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg               <  1:3034997/67‑1 (MQ=255)
                                                           ttCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATg           >  1:4252996/1‑71 (MQ=255)
                                                           ttCGGTCGGATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATg           >  1:214247/1‑71 (MQ=255)
                                                           ttCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACa                              >  1:1341354/1‑52 (MQ=255)
                                                            tcggtcggATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATgg          >  1:3898251/1‑71 (MQ=255)
                                                             cggtcggATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATgg          <  1:514963/70‑1 (MQ=255)
                                                             cggtcggATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATgg          >  1:961351/1‑70 (MQ=255)
                                                               gtcggATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATg               <  1:1388323/63‑1 (MQ=255)
                                                               gtcggATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCAt       <  1:131639/71‑1 (MQ=255)
                                                                tcggATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATc      <  1:212964/71‑1 (MQ=255)
                                                                tcggATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATc      <  1:4617694/71‑1 (MQ=255)
                                                                  ggATGATCGTTACCAAAAATCGCCAGCGAGTCGCGAc                                      <  1:496171/37‑1 (MQ=255)
                                                                  ggATGATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATCAg    >  1:4121867/1‑71 (MQ=255)
                                                                   gatgatCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCt                                 <  1:4269242/41‑1 (MQ=255)
                                                                    aTAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATCAGGt  <  1:2403119/71‑1 (MQ=255)
                                                                      |                                                                    
ACGACGTGACCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATCAGGT  >  minE/497777‑497915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A