Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R2
|
308 |
27.9 |
1975068 |
96.7% |
1909890 |
67.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,172,268 |
T→C |
100% |
K1092E (AAG→GAG) |
mfd ← |
transcription‑repair coupling factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,172,268 | 0 | T | C | 100.0%
| 18.8
/ NA
| 7 | K1092E (AAG→GAG) | mfd | transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (5/2); total (5/2) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATCCC > W3110S.gb/1172237‑1172322
|
ccAATCAACCAGGCCGGATTAACTTGATTCTCCTAGGCAAATTCGATCAccc > 1:1775989/1‑52 (MQ=255)
ccAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGa > 1:338559/1‑46 (MQ=255)
ccAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTc > 1:1687049/1‑71 (MQ=255)
ccAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTc > 1:204435/1‑71 (MQ=255)
ccAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTc > 1:930719/1‑71 (MQ=255)
ggATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATccc < 1:564499/71‑1 (MQ=255)
ggATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATccc < 1:699904/71‑1 (MQ=255)
|
CCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATCCC > W3110S.gb/1172237‑1172322
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
