Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I3 R2	122	64.1	3058059	97.6%	2984665	63.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,252,050	T→C	K6K (AAA→AAG)	yebC ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,252,050	0	T	C	100.0%	222.9 / NA	63	K6K (AAA→AAG)	yebC	conserved hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (29/34); total (29/34)

ATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTAC  >  minE/1251982‑1252113

aTGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCActt                                                               <  1:2117102/71‑1 (MQ=255)

 tGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTa                                                              >  1:1670783/1‑71 (MQ=255)

   aTTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACt                                                            <  1:1027056/71‑1 (MQ=255)

   aTTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACt                                                            <  1:1424727/71‑1 (MQ=255)

    ttttAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTa                                                           >  1:2895269/1‑71 (MQ=255)

    ttttAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTa                                                           >  1:2877071/1‑71 (MQ=255)

       tAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGa                                                        >  1:2633796/1‑71 (MQ=255)

       tAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGa                                                        >  1:1286770/1‑71 (MQ=255)

         gTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGAcc                                                      <  1:1024823/71‑1 (MQ=255)

            aaGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGc                                                   >  1:909784/1‑71 (MQ=255)

              gATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATg                                                         <  1:1347073/63‑1 (MQ=255)

              gATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATg                                                         <  1:1924819/63‑1 (MQ=255)

              gATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCa                                                 >  1:1657697/1‑71 (MQ=255)

              gATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCa                                                 >  1:1736791/1‑71 (MQ=255)

                ttttACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCAt                                                >  1:2479775/1‑70 (MQ=255)

                 tttACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTg                                                    >  1:1310831/1‑65 (MQ=255)

                     ccGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATaaaaa                                           <  1:2120824/70‑1 (MQ=255)

                      cgcgCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAata                                         <  1:1606802/71‑1 (MQ=255)

                          cTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATAtctc                                     <  1:2485448/71‑1 (MQ=255)

                          cTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGCCCCACTTACTATGACCTGCCATAAAAATAtctc                                     <  1:2105050/71‑1 (MQ=255)

                           ttAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATAtct                                      <  1:1252698/69‑1 (MQ=255)

                             aGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTcc                                    <  1:1504848/69‑1 (MQ=255)

                             aGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTcc                                    <  1:2425423/69‑1 (MQ=255)

                             aGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCa                                   <  1:2592948/70‑1 (MQ=255)

                             aGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCa                                   <  1:608283/70‑1 (MQ=255)

                              gCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATAtctc                                     >  1:1511908/1‑67 (MQ=255)

                              gCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAg                                  >  1:1885861/1‑70 (MQ=255)

                              gCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGa                                 <  1:2759517/71‑1 (MQ=255)

                              gCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGa                                 <  1:1076168/71‑1 (MQ=255)

                               cATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGAc                                                       >  1:479241/1‑48 (MQ=255)

                                aTCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATAtctc                                     <  1:1684142/65‑1 (MQ=255)

                                  ccTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGc                             >  1:1264804/1‑71 (MQ=255)

                                   cTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATaaaaa                                           >  1:1715972/1‑56 (MQ=255)

                                   cTGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATaaaaa                                           >  1:1606987/1‑56 (MQ=255)

                                    tGCGCAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATg                                                         <  1:1917737/41‑1 (MQ=255)

                                    tGCGCAGCTTTACGATGTCTGGTGTTAGCCCACTTACTATg                                                         <  1:1869938/41‑1 (MQ=255)

                                     gcgcAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAat                                          >  1:2152474/1‑55 (MQ=255)

                                      cgcAGCTTTACGATGTCTGGTGTTGGCCCACTTACTa                                                           <  1:1653548/37‑1 (MQ=255)

                                      cgcAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGAc                                                       >  1:1662249/1‑41 (MQ=255)

                                        cAGCTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATaa                                              <  1:2130672/48‑1 (MQ=255)

                                           cTTTACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGt                    <  1:1281609/71‑1 (MQ=255)

                                             ttACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAat                                          <  1:1040918/47‑1 (MQ=255)

                                             ttACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGcc                            >  1:2511285/1‑61 (MQ=255)

                                             ttACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGcc                            >  1:2562626/1‑61 (MQ=255)

                                             ttACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATATCCAGATAGcc                            >  1:287290/1‑61 (MQ=255)

                                             ttACGATGTCTGGTGTTGGCCCACTTACTATGAACTGCCATAAAAATATCTCCAGATAGCCCTGCCTGtt                   >  1:817994/1‑70 (MQ=255)

                                              tACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGtt                   >  1:347271/1‑69 (MQ=255)

                                              tACGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTc                  <  1:1031373/70‑1 (MQ=255)

                                                cGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCcct                          <  1:777730/60‑1 (MQ=255)

                                                cGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGtt                   <  1:1474489/67‑1 (MQ=255)

                                                cGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg               <  1:1970851/71‑1 (MQ=255)

                                                cGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg               <  1:2966693/71‑1 (MQ=255)

                                                cGATGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg               >  1:1382138/1‑71 (MQ=255)

                                                  aTGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCa             <  1:1629225/71‑1 (MQ=255)

                                                   tGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAg            >  1:2394891/1‑71 (MQ=255)

                                                   tGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAg            <  1:2153297/71‑1 (MQ=255)

                                                    gTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGc           >  1:2924571/1‑71 (MQ=255)

                                                       tGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGt         >  1:1374858/1‑70 (MQ=255)

                                                        ggTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGtt        >  1:2866766/1‑70 (MQ=255)

                                                            ttGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGAt                                <  1:2291244/42‑1 (MQ=255)

                                                            ttGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGAt                                <  1:1376904/42‑1 (MQ=255)

                                                            ttGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGtt        <  1:1392698/66‑1 (MQ=255)

                                                              ggCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTAc  >  1:3036276/1‑70 (MQ=255)

                                                                cccACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGtt                   <  1:2313116/51‑1 (MQ=255)

ATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTAC  >  minE/1251982‑1252113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A