Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
542,604 |
(T)6→5 |
coding (667/786 nt) |
ylbA ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 542,599 | 0 | T | . | 100.0%
| 183.9
/ NA
| 46 | coding (672/786 nt) | ylbA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (29/17); total (29/17) |
CACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGG > W3110S.gb/542550‑542660
|
cacCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGtt < 1:4164362/69‑1 (MQ=255)
cacCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGtt < 1:2099766/69‑1 (MQ=255)
cacCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTAt > 1:1490110/1‑71 (MQ=255)
cacCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTAt > 1:1495688/1‑71 (MQ=255)
cacCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTAt > 1:1660977/1‑71 (MQ=255)
cacCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTAt > 1:1873833/1‑71 (MQ=255)
aCCAGCCTGTAAAGAATAAGCGCTCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAg > 1:930783/1‑71 (MQ=255)
aCCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTAt > 1:2664261/1‑64 (MQ=255)
aCCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAg > 1:89246/1‑71 (MQ=255)
aCCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAg > 1:3531322/1‑71 (MQ=255)
aCCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAg > 1:2223235/1‑71 (MQ=255)
cAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGt < 1:2295868/71‑1 (MQ=255)
ccTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTta > 1:1201824/1‑70 (MQ=255)
ccTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTta > 1:33478/1‑70 (MQ=255)
ccTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTta > 1:1124843/1‑70 (MQ=255)
cTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCACTTATTGTCGAGGTtat > 1:2104183/1‑70 (MQ=255)
aaGAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAAccc > 1:2556458/1‑71 (MQ=255)
gAATAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAAccccc > 1:3092062/1‑71 (MQ=255)
aaTAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGt < 1:3155832/59‑1 (MQ=255)
tAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGa > 1:3479395/1‑71 (MQ=255)
tAAGCGCCCATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGa > 1:2930372/1‑71 (MQ=255)
cATAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAAcccc > 1:1960744/1‑59 (MQ=255)
tAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGt < 1:2751178/47‑1 (MQ=39)
tAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGt < 1:1951991/47‑1 (MQ=39)
tAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGaa < 1:2696885/71‑1 (MQ=255)
tAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGaa < 1:4219684/71‑1 (MQ=255)
tAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGaa < 1:276014/71‑1 (MQ=255)
tAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGaa < 1:88246/71‑1 (MQ=255)
tAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGaa < 1:1368826/71‑1 (MQ=255)
tAAAGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGaa < 1:39786/71‑1 (MQ=255)
aaGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAata < 1:502332/71‑1 (MQ=255)
aaGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAata > 1:1284634/1‑71 (MQ=255)
aaGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAata > 1:39640/1‑71 (MQ=255)
aaGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAata > 1:3283577/1‑71 (MQ=255)
aaGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAata < 1:1900386/71‑1 (MQ=255)
aaGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAata > 1:3343561/1‑71 (MQ=255)
aaGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAata < 1:2678320/71‑1 (MQ=255)
aaGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAata > 1:1969055/1‑71 (MQ=255)
aaGATGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAat > 1:1513521/1‑70 (MQ=255)
aaGATGTAATCGCC‑TTTTTCACAGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAata > 1:2708443/1‑71 (MQ=255)
tGTAATCGCC‑TTTTTCACCGGGATCCATTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAgg < 1:3107596/71‑1 (MQ=255)
tGTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGaa < 1:4326766/65‑1 (MQ=255)
gTAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAgg > 1:3733582/1‑70 (MQ=255)
tAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAAc > 1:2507327/1‑46 (MQ=39)
tAATCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAata > 1:4149062/1‑65 (MQ=255)
aTCGCC‑TTTTTCACCGGGATCCAGTTATTGTCGAGGTTATaaa > 1:2614730/1‑43 (MQ=38)
|
CACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGG > W3110S.gb/542550‑542660
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
