Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,849,128 |
G→T |
Q199K (CAG→AAG) |
selD ← |
selenophosphate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,849,128 | 0 | G | T | 100.0%
| 107.5
/ NA
| 37 | Q199K (CAG→AAG) | selD | selenophosphate synthase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (20/17); total (20/17) |
TTGGCAAAGGACGCGCCTGCGATGTTCATCCGGCACATCACTTCCGTCGCCAGTCCCTGATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAGAACGCCGATCCCCAGCGGTTTCGT > W3110S.gb/1849070‑1849194
|
ttGGCAAAGGACGCGCCTGCGATGTTCATCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGttt > 1:2035297/1‑71 (MQ=255)
ttGGCAAAGGACGCGCCTGCGATGTTCATCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGttt > 1:4198754/1‑71 (MQ=255)
ggCAAAGGACGCGCCTGCGATGTTCATCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTc < 1:1724413/70‑1 (MQ=255)
gCAAAGGACGCGCCTGCGATGTTCATCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCa < 1:3810942/70‑1 (MQ=255)
gCAAAGGACGCGCCTGCGATGTTCATCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCa < 1:3805510/70‑1 (MQ=255)
gACGCGCCTGCGATGTTCATCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGa > 1:3841813/1‑71 (MQ=255)
aTGTTCATCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGc > 1:828449/1‑71 (MQ=255)
gTTCATCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGcc > 1:1562747/1‑70 (MQ=255)
gTTCATCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCg > 1:2797522/1‑71 (MQ=255)
ttCATCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTc < 1:3889386/48‑1 (MQ=255)
ttCATCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTc < 1:2940122/48‑1 (MQ=255)
ttCATCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTc < 1:1562407/48‑1 (MQ=255)
tCATCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTg > 1:3193506/1‑71 (MQ=255)
cATCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTg > 1:1654599/1‑70 (MQ=255)
aTCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTgg < 1:2698699/70‑1 (MQ=255)
aTCCGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTgg < 1:4136165/70‑1 (MQ=255)
ccGGCACATCACTTCCGTCGCCAGTCCCTTATGtt > 1:832215/1‑35 (MQ=255)
ccGGCACATCACTTCCGTCGCCAGTCCCTTATGtt > 1:4050237/1‑35 (MQ=255)
cGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAg < 1:2508444/71‑1 (MQ=255)
cGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAg < 1:3624488/71‑1 (MQ=255)
cGGCACATCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAg < 1:1571586/71‑1 (MQ=255)
caTCACTTCCGTCGCCAGTCCCTTATGTTATGGTTTc < 1:3783085/37‑1 (MQ=37)
aTCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAGAACGcc < 1:357085/71‑1 (MQ=255)
aTCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAGAACGcc < 1:1344903/71‑1 (MQ=255)
tCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAGAACGcc > 1:4146303/1‑70 (MQ=255)
tCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAGAACGcc > 1:975507/1‑70 (MQ=255)
tCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAGAACGcc > 1:3306436/1‑70 (MQ=255)
tCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAGAACGcc > 1:3088426/1‑70 (MQ=255)
tCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAGAACGCCg < 1:684562/71‑1 (MQ=255)
tCACTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTAAGc > 1:265687/1‑55 (MQ=255)
aCTTCCGTCGCCAGTCCCTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAGAACGCCGAt > 1:256885/1‑71 (MQ=255)
cccTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGt > 1:4332148/1‑44 (MQ=255)
cccTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAGAACGCCGATCCCCAGCGGTTTCGt > 1:305323/1‑71 (MQ=255)
cccTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGT‑GTAAGAACGCCGATCCCCAGCGGTTTCGt > 1:1149067/1‑70 (MQ=255)
ccTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAg > 1:3960630/1‑36 (MQ=255)
ccTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAGAACGCCGATCCCCAGCGGTTTc < 1:623847/68‑1 (MQ=255)
ccTTATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAGAACGCCGATCCCCAGCGGTTTc < 1:1769735/68‑1 (MQ=255)
|
TTGGCAAAGGACGCGCCTGCGATGTTCATCCGGCACATCACTTCCGTCGCCAGTCCCTGATGTTCTGGTTTCAACAGTGATTTTTTCTCAGCCGTGGTAAGAACGCCGATCCCCAGCGGTTTCGT > W3110S.gb/1849070‑1849194
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
