Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R1
|
476 |
35.3 |
2812105 |
96.7% |
2719305 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,213,335 |
A→G |
10.6% |
N301S (AAT→AGT) |
yehQ → |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,213,335 | 0 | A | G | 10.6%
| 118.5
/ 7.3
| 47 | N301S (AAT→AGT) | yehQ | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (22/20); new base G (2/3); total (24/23) |
| Fisher's exact test for biased strand distribution p-value = 6.66e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 |
AGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACG > W3110S.gb/2213277‑2213399
|
agccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAaga < 1:2495170/71‑1 (MQ=255)
agccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAaga < 1:1517250/71‑1 (MQ=255)
gccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAGTTGCGCACAaga < 1:2723715/70‑1 (MQ=255)
gccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAaga < 1:2235232/70‑1 (MQ=255)
caCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAGTTGCGCACAAgaga > 1:550993/1‑70 (MQ=255)
aCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATg > 1:1604111/1‑71 (MQ=255)
cTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTaaa < 1:145308/54‑1 (MQ=255)
cTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTaaa < 1:317383/54‑1 (MQ=255)
tataATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTaaa < 1:842002/53‑1 (MQ=255)
ataATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAGTTGCGCACAAGAGATGGcc > 1:1770279/1‑71 (MQ=255)
ataATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAtaga > 1:1283272/1‑66 (MQ=255)
ataATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAgaga > 1:1147149/1‑66 (MQ=255)
ataATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGcc > 1:271907/1‑71 (MQ=255)
taATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCTCACAAGAGATGGc < 1:1752240/69‑1 (MQ=255)
taATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAaga < 1:2369883/63‑1 (MQ=255)
taATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAgaga > 1:345281/1‑65 (MQ=255)
taATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGccc > 1:2080124/1‑71 (MQ=255)
taATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGccc > 1:536173/1‑71 (MQ=255)
taATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGccc > 1:2344778/1‑71 (MQ=255)
gaATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCTACAg < 1:233170/71‑1 (MQ=255)
gaATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAg < 1:1480885/71‑1 (MQ=255)
aaTGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAagag < 1:1460065/53‑1 (MQ=255)
cTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTAtt > 1:692606/1‑70 (MQ=255)
tCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAg < 1:456034/71‑1 (MQ=255)
aGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATgg > 1:1334991/1‑45 (MQ=255)
aGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGt < 1:1957928/70‑1 (MQ=255)
gCTTGCGGCATTAAACAGTCGATTAAGTTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGa < 1:1050193/66‑1 (MQ=255)
gCTTGCGGCATTAAACAGTCGATTAAGTTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGa < 1:1570904/66‑1 (MQ=255)
cTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtcc < 1:2284120/71‑1 (MQ=255)
tGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTa > 1:1248655/1‑56 (MQ=255)
cATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCtgtg > 1:1045108/1‑71 (MQ=255)
ttAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTg < 1:2778818/55‑1 (MQ=255)
ttAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtcctc < 1:559828/64‑1 (MQ=255)
aaaCAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtc > 1:2760149/1‑59 (MQ=255)
aaaCAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtc > 1:555111/1‑59 (MQ=255)
aaaCAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGt > 1:2697716/1‑71 (MQ=255)
aaaCAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGt > 1:2537495/1‑71 (MQ=255)
aaaCAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGt > 1:825042/1‑71 (MQ=255)
aaaCAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGt > 1:1298802/1‑71 (MQ=255)
tCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGaa < 1:1697993/49‑1 (MQ=255)
tCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGaa < 1:1803719/49‑1 (MQ=255)
tCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGaa < 1:1376090/49‑1 (MQ=255)
cGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCAc > 1:1294076/1‑71 (MQ=255)
gATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtcctc > 1:2179288/1‑54 (MQ=255)
gATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACg > 1:1692512/1‑71 (MQ=255)
gATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACg < 1:2271195/71‑1 (MQ=255)
aTTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGtt < 1:396685/49‑1 (MQ=255)
aTTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGc < 1:502860/59‑1 (MQ=255)
ttAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACg < 1:2176476/69‑1 (MQ=255)
tAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtc > 1:2372672/1‑48 (MQ=255)
|
AGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACG > W3110S.gb/2213277‑2213399
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
