Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R1
|
476 |
35.3 |
2812105 |
96.7% |
2719305 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,712,033 |
G→A |
13.6% |
M328I (ATG→ATA) |
gidA → |
glucose‑inhibited cell‑division protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,712,033 | 0 | G | A | 13.6%
| 119.0
/ 9.7
| 44 | M328I (ATG→ATA) | gidA | glucose‑inhibited cell‑division protein |
| Reads supporting (aligned to +/- strand): ref base G (18/20); new base A (4/2); total (22/22) |
| Fisher's exact test for biased strand distribution p-value = 6.64e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 |
ATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCTC > W3110S.gb/3711963‑3712100
|
aTGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATg < 1:2731331/71‑1 (MQ=255)
aTGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATg > 1:2557779/1‑71 (MQ=255)
aTGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATg < 1:2168636/71‑1 (MQ=255)
tGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATg > 1:2142641/1‑70 (MQ=255)
aaaTTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAg > 1:844342/1‑71 (MQ=255)
aTTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAggg < 1:353741/71‑1 (MQ=255)
tttATCCGAACGGTATCACCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAggg > 1:741205/1‑70 (MQ=255)
cGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAg > 1:2673726/1‑58 (MQ=255)
tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAAcgcg > 1:261164/1‑66 (MQ=255)
tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAAcgcg > 1:2588299/1‑66 (MQ=255)
caccaGCCTTCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCg < 1:562605/71‑1 (MQ=255)
accaTCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGAt < 1:139612/68‑1 (MQ=255)
caGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGc > 1:1118948/1‑71 (MQ=255)
caGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGc > 1:293488/1‑71 (MQ=255)
caGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGc > 1:2544943/1‑71 (MQ=255)
caGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGc > 1:1785681/1‑71 (MQ=255)
aGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCg > 1:1530619/1‑71 (MQ=255)
aGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCg < 1:662479/71‑1 (MQ=255)
tGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCg > 1:1071157/1‑71 (MQ=255)
tGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCATCCg > 1:709130/1‑71 (MQ=255)
gCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATg < 1:1631593/37‑1 (MQ=255)
ccGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCggg > 1:2630739/1‑71 (MQ=255)
gATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGc < 1:1493362/71‑1 (MQ=255)
aTGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGcc < 1:1245105/71‑1 (MQ=255)
tgCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCAtt < 1:2719860/71‑1 (MQ=255)
aGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg < 1:990882/71‑1 (MQ=255)
aGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg < 1:234595/71‑1 (MQ=255)
aGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg < 1:926438/71‑1 (MQ=255)
aGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg < 1:2136671/71‑1 (MQ=255)
gATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg > 1:1574332/1‑70 (MQ=255)
tGCAAATCGTCCGCTCTTTGCAGGGGATGGAAAACGCGAAGAt < 1:1998100/43‑1 (MQ=255)
tGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGAt < 1:2621849/43‑1 (MQ=255)
gCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTAt > 1:175606/1‑70 (MQ=255)
aaaTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc < 1:1632698/71‑1 (MQ=255)
aaTCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTAt < 1:332897/67‑1 (MQ=255)
aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCg < 1:2656770/44‑1 (MQ=255)
aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCg < 1:1581078/44‑1 (MQ=255)
aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCg < 1:1988397/44‑1 (MQ=255)
aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGActt > 1:2609402/1‑71 (MQ=255)
aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGActt > 1:258362/1‑71 (MQ=255)
aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGActt > 1:2140957/1‑71 (MQ=255)
cGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGActtct > 1:1643034/1‑71 (MQ=255)
gTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTa > 1:1699367/1‑50 (MQ=255)
cGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCg < 1:887471/70‑1 (MQ=255)
cGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:317619/71‑1 (MQ=255)
cGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa > 1:26479/1‑71 (MQ=255)
ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:184125/43‑1 (MQ=255)
tATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAc < 1:589507/58‑1 (MQ=255)
tATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCTc > 1:1811092/1‑71 (MQ=255)
|
ATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCTC > W3110S.gb/3711963‑3712100
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
