Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R2
|
232 |
76.3 |
4135488 |
90.9% |
3759158 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,094,567 |
T→C |
33.3% |
I604V (ATT→GTT) |
ydiJ ← |
predicted FAD‑linked oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,094,567 | 0 | T | C | 33.3%
| 62.4
/ 52.4
| 57 | I604V (ATT→GTT) | ydiJ | predicted FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (20/18); new base C (10/9); total (30/27) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 |
GCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGT > minE/1094502‑1094635
|
gccgcGATCCGCCAACAAACGCAGCCATTCACGCCCCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGt > 1:3110066/1‑71 (MQ=255)
gccgcGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGt > 1:3010894/1‑71 (MQ=255)
gccgcGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGt > 1:3442040/1‑71 (MQ=255)
cgcgATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTc < 1:3589212/71‑1 (MQ=255)
cgcgATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTc < 1:2244312/71‑1 (MQ=255)
cgcgATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTc < 1:3794971/71‑1 (MQ=255)
cgcgATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTc < 1:2121667/71‑1 (MQ=255)
ccGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGt > 1:1474944/1‑70 (MQ=255)
cGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTg < 1:1300538/70‑1 (MQ=255)
cGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTg < 1:3778496/70‑1 (MQ=255)
aaaCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCAt > 1:2952103/1‑71 (MQ=255)
aaaCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCAt > 1:2285726/1‑71 (MQ=255)
aaaCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCAt > 1:2143816/1‑71 (MQ=255)
aaaCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCAt > 1:1487982/1‑71 (MQ=255)
aaCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATc < 1:769142/71‑1 (MQ=255)
aaCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATc < 1:1838316/71‑1 (MQ=255)
gCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTg < 1:3124679/59‑1 (MQ=255)
gCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGa < 1:3821289/71‑1 (MQ=255)
gCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGa < 1:4093009/71‑1 (MQ=255)
cATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGaca < 1:3592776/71‑1 (MQ=255)
tCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACAt > 1:4013628/1‑71 (MQ=255)
tCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACAt > 1:2711837/1‑71 (MQ=255)
cACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATc > 1:3791646/1‑71 (MQ=255)
cACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATc > 1:2252052/1‑71 (MQ=255)
cACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATc > 1:2748763/1‑71 (MQ=255)
aCGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCg < 1:1640670/71‑1 (MQ=255)
aCGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCg < 1:1044575/71‑1 (MQ=255)
aCGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCg < 1:2361188/71‑1 (MQ=255)
gCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCgg < 1:1892313/70‑1 (MQ=255)
cAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTAc < 1:995342/71‑1 (MQ=255)
cAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTAc < 1:881642/71‑1 (MQ=255)
cAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTAc < 1:386748/71‑1 (MQ=255)
aGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACg > 1:547268/1‑71 (MQ=255)
aGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACg > 1:3070344/1‑71 (MQ=255)
aGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACg > 1:4098458/1‑71 (MQ=255)
gCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTc > 1:47764/1‑47 (MQ=255)
tGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCAt > 1:1403616/1‑71 (MQ=255)
gcgcgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCgg > 1:2279447/1‑59 (MQ=255)
gcgcgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGa > 1:3682355/1‑60 (MQ=255)
gcgcgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGa > 1:2473337/1‑60 (MQ=255)
gcgcgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGa > 1:2200209/1‑60 (MQ=255)
cgcgcCCTTTCTGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCa < 1:3936564/71‑1 (MQ=255)
cgcgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATc < 1:2543157/70‑1 (MQ=255)
cgcgcCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCa < 1:1350096/71‑1 (MQ=255)
cgcgcCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCa < 1:893668/71‑1 (MQ=255)
cgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCaaa < 1:693901/71‑1 (MQ=255)
cgcCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCAtaa > 1:1624598/1‑67 (MQ=255)
cTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTg < 1:3064006/71‑1 (MQ=255)
cTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTg < 1:586221/71‑1 (MQ=255)
tCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTa > 1:3630044/1‑53 (MQ=255)
tCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGaa > 1:1157582/1‑70 (MQ=255)
tCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAg > 1:390214/1‑71 (MQ=255)
tCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAg > 1:3425914/1‑71 (MQ=255)
tCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAg > 1:293875/1‑71 (MQ=255)
tCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAg > 1:2122327/1‑71 (MQ=255)
tCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAg > 1:108809/1‑71 (MQ=255)
ggTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGc < 1:3390226/70‑1 (MQ=255)
aatgaatCCGGTTCTGGGTGATCTTCATCGACGGACACATCg < 1:4125754/42‑1 (MQ=255)
aatgaatCCGGTTCTGGGTGAGCTTCATCGACGGACACATCg < 1:385002/42‑1 (MQ=255)
aatCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGt < 1:2370766/71‑1 (MQ=255)
aatCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGt < 1:2126987/71‑1 (MQ=255)
aatCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGt < 1:1991017/71‑1 (MQ=255)
|
GCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGT > minE/1094502‑1094635
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
