Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R2
|
232 |
76.3 |
4135488 |
90.9% |
3759158 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,257,376 |
T→C |
13.0% |
F7L (TTT→CTT) |
yecP → |
predicted methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,257,376 | 0 | T | C | 13.0%
| 200.8
/ 19.8
| 77 | F7L (TTT→CTT) | yecP | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (31/36); new base C (2/8); total (33/44) |
| Fisher's exact test for biased strand distribution p-value = 1.74e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTG > minE/1257307‑1257446
|
ccAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACtt > 1:925010/1‑71 (MQ=255)
ccAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACtt > 1:816527/1‑71 (MQ=255)
cAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACttt < 1:2735571/71‑1 (MQ=255)
cAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACttt < 1:3580764/71‑1 (MQ=255)
cAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACttt < 1:1462675/71‑1 (MQ=255)
aGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACttt > 1:3723678/1‑70 (MQ=255)
gTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTa < 1:4110750/71‑1 (MQ=255)
tGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACtt > 1:1754632/1‑67 (MQ=255)
tGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACtt > 1:1048857/1‑67 (MQ=255)
tGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTAt < 1:2819500/71‑1 (MQ=255)
tGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTAt < 1:260184/71‑1 (MQ=255)
tGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTAt < 1:1780242/71‑1 (MQ=255)
gCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACttt < 1:2348311/67‑1 (MQ=255)
cTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATtc > 1:4106718/1‑71 (MQ=255)
cTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATtc > 1:2257996/1‑71 (MQ=255)
aaCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTctct < 1:288201/70‑1 (MQ=255)
aCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTctct < 1:3549258/69‑1 (MQ=255)
ttGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTctct < 1:120175/66‑1 (MQ=255)
ggTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGc > 1:2470649/1‑70 (MQ=255)
cACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCaaa > 1:838876/1‑70 (MQ=255)
aCTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGCTAACTTTTa < 1:3044617/53‑1 (MQ=255)
ggtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATtc < 1:1236698/53‑1 (MQ=255)
ggtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTctct < 1:1750163/56‑1 (MQ=255)
gtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATtct < 1:3112947/53‑1 (MQ=255)
gtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATtct < 1:1536368/53‑1 (MQ=255)
gtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATtct < 1:2228837/53‑1 (MQ=255)
gtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAatc < 1:3626723/69‑1 (MQ=255)
ggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTctct > 1:1145980/1‑53 (MQ=255)
ggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCt > 1:361394/1‑71 (MQ=255)
gCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACctt < 1:3293534/44‑1 (MQ=255)
gCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACctt < 1:383499/44‑1 (MQ=255)
gCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACctt < 1:1756648/44‑1 (MQ=255)
gCATTAAAAGCAGAGGACGCTCCATGATCGACTTTTGTAACctt < 1:214632/44‑1 (MQ=38)
cATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:1726009/71‑1 (MQ=255)
cATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:3945364/71‑1 (MQ=255)
ttAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:1863548/69‑1 (MQ=255)
ttAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:2485697/69‑1 (MQ=255)
tAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTca > 1:3953559/1‑70 (MQ=255)
aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACtt > 1:319264/1‑38 (MQ=255)
aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:3527549/1‑71 (MQ=255)
aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:1414576/1‑71 (MQ=255)
aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:1461827/1‑71 (MQ=255)
aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:2407644/1‑71 (MQ=255)
aaaaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:313938/1‑71 (MQ=255)
gagGACGCTGCTTGATCGACTTTGGTATGTTTTAt < 1:906330/35‑1 (MQ=25)
ggACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGa < 1:1585102/40‑1 (MQ=255)
ggACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGa < 1:3643891/71‑1 (MQ=255)
gACGCTGCATGATCGACTTTGGTAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGa < 1:937862/70‑1 (MQ=255)
tGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt > 1:2219788/1‑52 (MQ=255)
tGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt > 1:862631/1‑52 (MQ=255)
tGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt > 1:1723460/1‑52 (MQ=255)
gCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTca > 1:2503168/1‑53 (MQ=255)
gCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTca > 1:3557578/1‑53 (MQ=255)
tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaaa < 1:1798883/38‑1 (MQ=255)
tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaaa < 1:1226324/38‑1 (MQ=255)
tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaaa < 1:1305596/38‑1 (MQ=255)
tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaaa < 1:3652085/38‑1 (MQ=255)
tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaaa < 1:1792782/38‑1 (MQ=255)
tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaaa < 1:3873886/38‑1 (MQ=255)
tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaaa < 1:2278843/38‑1 (MQ=255)
tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAatcat < 1:410757/44‑1 (MQ=255)
aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCt > 1:3128820/1‑44 (MQ=255)
aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCt > 1:2354633/1‑44 (MQ=255)
aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcgc > 1:738914/1‑71 (MQ=255)
aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcgc > 1:423041/1‑71 (MQ=255)
aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcgc > 1:2970144/1‑71 (MQ=255)
aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcgc > 1:1212705/1‑71 (MQ=255)
aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcgc > 1:1907653/1‑71 (MQ=255)
aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcgc > 1:2702683/1‑71 (MQ=255)
aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcg > 1:874116/1‑70 (MQ=255)
aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCcg > 1:838539/1‑70 (MQ=255)
aTCGACTTTGGTAACTTTTATTCTATGATTGCCAAAAatcat > 1:51322/1‑42 (MQ=255)
tCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAatcat < 1:447498/41‑1 (MQ=255)
tCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:870736/45‑1 (MQ=255)
tCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:1772089/45‑1 (MQ=255)
gACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:342719/71‑1 (MQ=255)
gACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:2040894/71‑1 (MQ=255)
tttGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:1720377/40‑1 (MQ=255)
tGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:3060878/1‑42 (MQ=255)
tGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:4092567/1‑42 (MQ=255)
tGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca > 1:868536/1‑42 (MQ=255)
tGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcac > 1:3170983/1‑41 (MQ=255)
tGGTAACTTTTATTCTCTGATTGACAAAAATCATCTTTcaca > 1:875191/1‑42 (MQ=255)
gTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCt < 1:1462119/71‑1 (MQ=255)
gTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCt < 1:1113790/71‑1 (MQ=255)
tAACCTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTa < 1:3857218/71‑1 (MQ=255)
gaaCTTTTATTCTCTGATTGCCAAAAATCATCttt < 1:2682795/34‑1 (MQ=255)
aCTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAAc < 1:2773737/71‑1 (MQ=255)
aCTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAAc < 1:3464387/71‑1 (MQ=255)
aCTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAAc < 1:3661786/71‑1 (MQ=255)
cTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACt < 1:3558670/71‑1 (MQ=255)
ttttATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:683280/59‑1 (MQ=255)
ttttATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa < 1:2138883/59‑1 (MQ=255)
ttttATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTg > 1:2336597/1‑71 (MQ=255)
|
CCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTG > minE/1257307‑1257446
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
