Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F3 I0 R2 232 76.3 4135488 90.9% 3759158 60.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,211,683 A→G 30.1% I465I (ATT→ATC)  rng ← ribonuclease G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,211,6830AG30.1% 99.0 / 61.7 73I465I (ATT→ATC) rngribonuclease G
Reads supporting (aligned to +/- strand):  ref base A (29/22);  new base G (9/13);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-01

CATTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCA  >  minE/2211614‑2211749
                                                                     |                                                                  
catTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGaaaa                                                                   >  1:3430115/1‑71 (MQ=255)
   tACGACGTCAAACTGCTCCTTGTTATAGAGCGGTTCAATTTGTACTTTATCCTGTTTGCCAACGAAAAtt                                                                 <  1:2953527/70‑1 (MQ=255)
     cgacgTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTc                                                               <  1:3363420/70‑1 (MQ=255)
     cgacgTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTc                                                               <  1:192059/70‑1 (MQ=255)
     cgacgTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTc                                                               <  1:1887999/70‑1 (MQ=255)
            aaaCTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTcc                                                        >  1:2670330/1‑70 (MQ=255)
            aaaCTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCg                                                       >  1:1422034/1‑71 (MQ=255)
            aaaCTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCAc                                                            >  1:2437360/1‑66 (MQ=255)
            aaaCTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCg                                                       >  1:2143359/1‑71 (MQ=255)
            aaaCTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCg                                                       >  1:3202756/1‑71 (MQ=255)
            aaaCTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCg                                                       >  1:2697094/1‑71 (MQ=255)
            aaaCTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCg                                                       >  1:1857065/1‑71 (MQ=255)
            aaaCTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCg                                                       >  1:563058/1‑71 (MQ=255)
            aaaCTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCg                                                       >  1:1202212/1‑71 (MQ=255)
            aaaCTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCAAACGAAAATTTCCAc                                                            >  1:101870/1‑66 (MQ=255)
                tGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACt                                                           >  1:164476/1‑63 (MQ=255)
                     cTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAgt                                              <  1:1549957/71‑1 (MQ=255)
                        gTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAgtgt                                            <  1:3415245/70‑1 (MQ=255)
                        gTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAgtgt                                            <  1:1999469/70‑1 (MQ=255)
                        gTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgtgt                                            <  1:3395430/70‑1 (MQ=255)
                        gTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgtgt                                            <  1:1737836/70‑1 (MQ=255)
                         ttATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAGTGTGa                                          <  1:191573/71‑1 (MQ=255)
                         ttATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAGTGTGa                                          <  1:551202/71‑1 (MQ=255)
                         ttATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAGTGTGa                                          <  1:817869/71‑1 (MQ=255)
                         ttATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGa                                          <  1:3797660/71‑1 (MQ=255)
                         ttATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGa                                          <  1:1503825/71‑1 (MQ=255)
                          tataGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGAc                                         >  1:69036/1‑71 (MQ=255)
                            taGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAGTGTGActc                                       <  1:252970/71‑1 (MQ=255)
                                 cGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGc                                  <  1:4059848/71‑1 (MQ=255)
                                  ggTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGc                                  <  1:27116/70‑1 (MQ=255)
                                   gTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCAc                                                            >  1:1926247/1‑43 (MQ=255)
                                    ttCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTc                                    <  1:3171595/66‑1 (MQ=255)
                                     tCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCttt                              >  1:2233714/1‑71 (MQ=255)
                                     tCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCttt                              >  1:2504605/1‑71 (MQ=255)
                                      cAATTTGTACTTTAACCTGTTTGGCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCttt                              >  1:2995046/1‑70 (MQ=255)
                                      cAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTc                                                               <  1:420357/37‑1 (MQ=255)
                                      cAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCttt                              >  1:3151792/1‑70 (MQ=255)
                                      cAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCttt                              >  1:3397673/1‑70 (MQ=255)
                                      cAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCttt                              >  1:167231/1‑70 (MQ=255)
                                      cAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCttt                              >  1:3469630/1‑70 (MQ=255)
                                      cAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCttt                              >  1:1276480/1‑70 (MQ=255)
                                      cAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCttt                              >  1:1844630/1‑70 (MQ=255)
                                      cAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCAGCCAGCGAGTGTGACTCTTCGCCttt                              >  1:2350655/1‑70 (MQ=255)
                                      cAATTTGTACTTTAACATGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCttt                              >  1:3819617/1‑70 (MQ=255)
                                       aaTTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTc                             >  1:2352695/1‑70 (MQ=255)
                                       aaTTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTc                             >  1:3555646/1‑70 (MQ=255)
                                       aaTTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCa                            <  1:783475/71‑1 (MQ=255)
                                       aaTTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCa                            <  1:3048326/71‑1 (MQ=255)
                                         tttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAg                          <  1:2026746/71‑1 (MQ=255)
                                         tttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAg                          <  1:1953538/71‑1 (MQ=255)
                                         tttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAg                          <  1:1655095/71‑1 (MQ=255)
                                         tttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAg                          <  1:1113610/71‑1 (MQ=255)
                                                 ttAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGc                  >  1:271526/1‑71 (MQ=255)
                                                 ttAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGc                  >  1:161915/1‑71 (MQ=255)
                                                 ttAACCTCTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGc                  >  1:721732/1‑71 (MQ=255)
                                                  tAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGc                  <  1:880810/70‑1 (MQ=255)
                                                  tAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGc                  >  1:3399300/1‑70 (MQ=255)
                                                  tAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGc                  <  1:2674482/70‑1 (MQ=255)
                                                  tAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGc                  >  1:2654493/1‑70 (MQ=255)
                                                  tAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGc                  >  1:1166305/1‑70 (MQ=255)
                                                    aCCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt                 <  1:1769678/69‑1 (MQ=255)
                                                         tttGCCAACGAAGATTTCCACTTCCGCCAGCGAGtgtg                                           <  1:3692679/38‑1 (MQ=255)
                                                           tGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGa                                          >  1:1299917/1‑37 (MQ=255)
                                                               aaCGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAg    <  1:3175612/71‑1 (MQ=255)
                                                               aaCGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAg    <  1:3933790/71‑1 (MQ=255)
                                                                aCGAAGATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAg    >  1:2465372/1‑70 (MQ=255)
                                                                aCGAAGATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAg    >  1:2652383/1‑70 (MQ=255)
                                                                aCGAAGATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAg    >  1:1727996/1‑70 (MQ=255)
                                                                aCGAAGATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAg    >  1:1269678/1‑70 (MQ=255)
                                                                aCGAAGATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAg    >  1:1212766/1‑70 (MQ=255)
                                                                aCGAAGATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGc   <  1:569410/71‑1 (MQ=255)
                                                                aCGAAGATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGc   <  1:75753/71‑1 (MQ=255)
                                                                 cGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCa  <  1:2066187/71‑1 (MQ=255)
                                                                 cGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCa  <  1:3910212/71‑1 (MQ=255)
                                                                   aaaaTTTCCACTTCCGCCAGCGAGTGTGACTCATCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCa  >  1:45630/1‑69 (MQ=255)
                                                                     |                                                                  
CATTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCA  >  minE/2211614‑2211749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A