Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R2
|
278 |
43.2 |
2215644 |
94.6% |
2095999 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,211,683 |
A→G |
18.6% |
I465I (ATT→ATC) |
rng ← |
ribonuclease G |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,211,683 | 0 | A | G | 18.6%
| 91.8
/ 17.3
| 43 | I465I (ATT→ATC) | rng | ribonuclease G |
| Reads supporting (aligned to +/- strand): ref base A (20/15); new base G (4/4); total (24/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCATTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCATA > minE/2211613‑2211751
|
tcatTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGaaa < 1:404652/71‑1 (MQ=255)
tcatTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGaaa < 1:450382/71‑1 (MQ=255)
tcatTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGaaa < 1:1351598/71‑1 (MQ=255)
catTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGaaaa > 1:517866/1‑71 (MQ=255)
atTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTGCTTTAACCTGTTTGCCAACGAAAAt > 1:115778/1‑71 (MQ=255)
atTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAAt > 1:1462552/1‑71 (MQ=255)
aaaCTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCAc > 1:197548/1‑66 (MQ=255)
aaaCTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTcc > 1:1247890/1‑70 (MQ=255)
cTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTc > 1:1214750/1‑60 (MQ=255)
cTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGaaa < 1:91193/49‑1 (MQ=255)
cTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgt > 1:1183196/1‑71 (MQ=255)
tGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAgtg > 1:356843/1‑71 (MQ=255)
ggTTATAGAGCGGTTCAATTTTTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgtgt > 1:115365/1‑71 (MQ=255)
gTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAgtgt < 1:1535818/70‑1 (MQ=255)
gTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAGtgtg < 1:1443985/71‑1 (MQ=255)
gTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgtgt < 1:833834/70‑1 (MQ=255)
ttATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGAtt < 1:1027135/48‑1 (MQ=255)
ataGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGaaaa > 1:1391474/1‑44 (MQ=255)
ataGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCa > 1:919867/1‑59 (MQ=255)
agagCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCAc > 1:1377906/1‑49 (MQ=255)
gagCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCtt < 1:867178/71‑1 (MQ=255)
tCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAGTGTGACACTTCGCCttt > 1:1186360/1‑71 (MQ=255)
cAATTTGTACTTTAACCTGTTTGCCAACGAAAAttt > 1:1423090/1‑36 (MQ=255)
aaTTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTc < 1:835020/70‑1 (MQ=255)
aTTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGa > 1:648828/1‑50 (MQ=255)
tttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTcc < 1:1935384/41‑1 (MQ=255)
tttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgtgt < 1:1161618/53‑1 (MQ=255)
ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAgg > 1:1329379/1‑71 (MQ=255)
tttAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGac < 1:1835361/43‑2 (MQ=255)
tAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAgtg > 1:973805/1‑43 (MQ=255)
tAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgtg > 1:1557005/1‑43 (MQ=255)
aCCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGc < 1:2037415/68‑1 (MQ=255)
aCCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTAc > 1:161247/1‑71 (MQ=255)
cTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgtgt < 1:923377/40‑1 (MQ=255)
cTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgtgt < 1:2016227/40‑1 (MQ=255)
gTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGa > 1:2133015/1‑40 (MQ=255)
gTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGa > 1:457207/1‑40 (MQ=255)
tttGCCAACGAAAATTTCCACTTCCGCCAGCGAGtgtg < 1:1994825/38‑1 (MQ=255)
tttGCCAACGAAAATTTCCACTTCCGCCAGCGAGtgtg < 1:78434/38‑1 (MQ=255)
tttGCCAACGAAAATTTCCACTTCCGCCAGCGAGtgtg < 1:1217101/38‑1 (MQ=255)
tttGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCg < 1:741098/71‑1 (MQ=255)
gCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGga < 1:1687427/70‑1 (MQ=255)
ccAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGga < 1:2021039/69‑1 (MQ=255)
aaCGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAg > 1:2054573/1‑71 (MQ=255)
aaCGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAg > 1:1251650/1‑71 (MQ=255)
aCGAAGATTTCCACTTCAGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGc < 1:1949891/71‑1 (MQ=255)
gAAGATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCa > 1:1394016/1‑70 (MQ=255)
gAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCa > 1:123711/1‑70 (MQ=255)
gAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCa > 1:1678930/1‑70 (MQ=255)
aaaaTTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCATa < 1:681289/71‑1 (MQ=255)
|
TCATTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCATA > minE/2211613‑2211751
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
