Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R2
|
232 |
76.3 |
4135488 |
90.9% |
3759158 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,236,903 |
G→A |
27.7% |
A150T (GCT→ACT) |
purD → |
phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,236,903 | 0 | G | A | 27.7%
| 108.4
/ 45.9
| 65 | A150T (GCT→ACT) | purD | phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase |
| Reads supporting (aligned to +/- strand): ref base G (27/20); new base A (7/11); total (34/31) |
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 |
ACCTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGATATGCTGGC > minE/2236842‑2236973
|
aCCTGCGCTGGCGTATCTGCGTGAGAAAGTCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGa < 1:3042397/71‑1 (MQ=255)
aCCTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGa < 1:1098481/71‑1 (MQ=255)
ccTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGa > 1:2167761/1‑70 (MQ=255)
ccTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGa > 1:787939/1‑70 (MQ=255)
gCGTATCTGCGTGAGAAAGGCGGGCCAATCGTCATTAAGGCGGACGGTCTGGCTGCCGGGaaa > 1:669681/1‑63 (MQ=255)
gCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGaaa > 1:3328702/1‑63 (MQ=255)
gCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTAt > 1:3436195/1‑71 (MQ=255)
gCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTAt > 1:2042696/1‑71 (MQ=255)
gCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTAt > 1:3482935/1‑71 (MQ=255)
tGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGtt < 1:331035/62‑1 (MQ=255)
tGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGtt < 1:412297/62‑1 (MQ=255)
tGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGc > 1:2671843/1‑70 (MQ=255)
tGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGc > 1:435384/1‑70 (MQ=255)
cGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGc < 1:841690/68‑1 (MQ=255)
cGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGc < 1:1143134/68‑1 (MQ=255)
cGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGc < 1:2311075/68‑1 (MQ=255)
cGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGc < 1:2202056/68‑1 (MQ=255)
cGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGc < 1:2165099/68‑1 (MQ=255)
cGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGAt < 1:2239992/71‑1 (MQ=255)
cGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGAt < 1:2155433/71‑1 (MQ=255)
cGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGAt < 1:3714704/71‑1 (MQ=255)
cGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGAt < 1:3935053/71‑1 (MQ=255)
tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGa > 1:1958875/1‑71 (MQ=255)
tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGa > 1:2657215/1‑71 (MQ=255)
tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGa > 1:1436898/1‑71 (MQ=255)
gagaAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGa < 1:2479587/70‑1 (MQ=255)
aGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTgg < 1:3385566/71‑1 (MQ=255)
aGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTgg < 1:1800926/71‑1 (MQ=255)
cgcgcCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaag < 1:2965709/71‑1 (MQ=255)
cgcCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaaga < 1:1701084/70‑1 (MQ=255)
aTCGTCATTAAAGCGGACGGTCTGGCTGCCGGGaaa < 1:2567473/36‑1 (MQ=255)
tCGTCATTAAAGCTGACGGTCTGGCTGCCGGGaaa < 1:3482463/35‑1 (MQ=255)
tCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTAt < 1:3051856/43‑1 (MQ=255)
cGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaag > 1:3292206/1‑71 (MQ=255)
cGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaa > 1:926289/1‑70 (MQ=255)
gTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTAt < 1:1046668/41‑1 (MQ=255)
gTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCg > 1:3634237/1‑43 (MQ=255)
gTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCg > 1:1544179/1‑43 (MQ=255)
gTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATg > 1:3217465/1‑51 (MQ=255)
gTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATg > 1:1468003/1‑51 (MQ=255)
gTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATg > 1:235601/1‑51 (MQ=255)
gTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATg > 1:2405440/1‑51 (MQ=255)
gTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagc > 1:279236/1‑71 (MQ=255)
gTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagc > 1:2046206/1‑71 (MQ=255)
gTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagc > 1:2338066/1‑71 (MQ=255)
gTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGAGTTATCg > 1:2035120/1‑43 (MQ=255)
gTCATAAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATg > 1:2108892/1‑51 (MQ=255)
tCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGtt < 1:1215150/38‑1 (MQ=255)
tCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGtt < 1:868098/38‑1 (MQ=255)
tCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGtt < 1:2899263/38‑1 (MQ=255)
tCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGtt < 1:4021/38‑1 (MQ=255)
tCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGtt < 1:37305/38‑1 (MQ=255)
tCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGtt < 1:3814224/38‑1 (MQ=255)
cATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:4118916/1‑70 (MQ=255)
cATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGaagcggaagcgg > 1:3002319/1‑71 (MQ=255)
aaaGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGt > 1:2496986/1‑71 (MQ=255)
gACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGa < 1:2042139/71‑1 (MQ=255)
gACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGa < 1:2487637/71‑1 (MQ=255)
gACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGa < 1:3126265/71‑1 (MQ=255)
gACGGTATGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGa > 1:531738/1‑71 (MQ=255)
cGGTCTGGCTGCCGGGAAAGGCGTTATCGTGTCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGata > 1:3164264/1‑71 (MQ=255)
tGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGATATGCTg > 1:1865823/1‑71 (MQ=255)
tGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGATATGCTg > 1:448438/1‑71 (MQ=255)
tGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGATATGCTg > 1:3237202/1‑71 (MQ=255)
tGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGATATGCTg > 1:2600266/1‑71 (MQ=255)
gCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGATATGCt < 1:808172/68‑1 (MQ=255)
gCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGATATGCTGGc > 1:1515742/1‑71 (MQ=255)
|
ACCTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGATATGCTGGC > minE/2236842‑2236973
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
