Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R2
|
296 |
77.6 |
4260404 |
88.1% |
3753415 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,236,903 |
G→A |
45.9% |
A150T (GCT→ACT) |
purD → |
phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,236,903 | 0 | G | A | 45.9%
| 19.2
/ 81.4
| 61 | A150T (GCT→ACT) | purD | phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase |
| Reads supporting (aligned to +/- strand): ref base G (20/13); new base A (18/10); total (38/23) |
| Fisher's exact test for biased strand distribution p-value = 7.97e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTAGAACCTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGATATGCT > minE/2236837‑2236970
|
gTAGAACCTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGc > 1:2264255/1‑71 (MQ=255)
aGAACCTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCg > 1:3185880/1‑71 (MQ=255)
aCCTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGa < 1:777273/71‑1 (MQ=255)
ccTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGaa < 1:3185941/71‑1 (MQ=255)
ccTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGa > 1:153489/1‑70 (MQ=255)
cgcTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAg < 1:4009243/69‑1 (MQ=255)
gCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGtt < 1:1725880/69‑1 (MQ=255)
cGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATc < 1:1248767/71‑1 (MQ=255)
cGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATc < 1:2476028/71‑1 (MQ=255)
aTCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGAGTGCCGGGa < 1:366115/57‑1 (MQ=255)
tCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAgg < 1:2672240/60‑1 (MQ=255)
tCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTgg > 1:3117701/1‑71 (MQ=255)
tCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTgg > 1:3839979/1‑71 (MQ=255)
tCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTgg > 1:1775064/1‑71 (MQ=255)
tCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGa < 1:670178/56‑1 (MQ=255)
tGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCg > 1:1497393/1‑66 (MQ=255)
gCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGa < 1:747367/53‑1 (MQ=255)
cGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGc < 1:1016544/68‑1 (MQ=255)
cGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGc < 1:2485555/68‑1 (MQ=255)
agaAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGa > 1:3731541/1‑66 (MQ=255)
aaaGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCt > 1:790524/1‑71 (MQ=255)
aaaGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTTCCGGGAAAGGCg > 1:1987108/1‑52 (MQ=255)
aaaGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCg > 1:1086079/1‑52 (MQ=255)
gcgcgcCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGaaa > 1:812703/1‑44 (MQ=255)
aaTCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcg > 1:2270006/1‑70 (MQ=255)
aTCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTAt > 1:1954187/1‑44 (MQ=255)
aTCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTAt > 1:1426525/1‑44 (MQ=255)
aTCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTAt > 1:3061269/1‑44 (MQ=255)
tCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGAt < 1:1535155/52‑1 (MQ=255)
tCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGAt < 1:1530242/52‑1 (MQ=255)
tCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTg < 1:4051871/59‑1 (MQ=255)
tCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTg < 1:3760827/59‑1 (MQ=255)
cGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGa > 1:536630/1‑53 (MQ=255)
cGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGa > 1:2039352/1‑53 (MQ=255)
tCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACg < 1:516141/53‑1 (MQ=255)
tCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACg < 1:967498/53‑1 (MQ=255)
tCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACg < 1:2863338/53‑1 (MQ=255)
tCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg < 1:2685062/71‑1 (MQ=255)
cATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGaagcggaagcgg > 1:2017247/1‑71 (MQ=255)
cATTAAAGCGGAAGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGaagcggaagcgg > 1:1598905/1‑71 (MQ=255)
aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGc > 1:741351/1‑71 (MQ=255)
tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaagaa < 1:858603/58‑1 (MQ=255)
tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTg > 1:1039613/1‑71 (MQ=255)
tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTg > 1:1710888/1‑71 (MQ=255)
tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTg > 1:617218/1‑71 (MQ=255)
tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTg > 1:3245023/1‑71 (MQ=255)
tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTg > 1:914242/1‑71 (MQ=255)
aaaGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGa > 1:4250096/1‑46 (MQ=255)
aaaGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGa > 1:269238/1‑46 (MQ=255)
aaaGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGa > 1:1806436/1‑46 (MQ=255)
aaaGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGa > 1:3829756/1‑46 (MQ=255)
aaaGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGt > 1:2567528/1‑71 (MQ=255)
aaaGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGt > 1:3874952/1‑71 (MQ=255)
aaGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGtt < 1:690621/71‑1 (MQ=255)
gACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGa > 1:524953/1‑71 (MQ=255)
gACGGTCTGGCTGCCGGGAAAGGCGTTATCGTAGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGa > 1:649557/1‑71 (MQ=255)
tCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGATATGc > 1:2859478/1‑71 (MQ=255)
tCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGATATGc > 1:1514422/1‑71 (MQ=255)
gcTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGATATGc < 1:2267709/70‑1 (MQ=255)
cTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGATATGCt > 1:4005696/1‑71 (MQ=255)
cTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGATATGCt > 1:3107326/1‑71 (MQ=255)
|
GTAGAACCTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCGGCTGTTCACGATATGCT > minE/2236837‑2236970
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
