Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R2
|
232 |
76.3 |
4135488 |
90.9% |
3759158 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,687,159 |
G→A |
26.2% |
L374L (CTG→TTG) |
pck ← |
phosphoenolpyruvate carboxykinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,687,159 | 0 | G | A | 26.2%
| 152.3
/ 57.1
| 84 | L374L (CTG→TTG) | pck | phosphoenolpyruvate carboxykinase |
| Reads supporting (aligned to +/- strand): ref base G (29/33); new base A (12/10); total (41/43) |
| Fisher's exact test for biased strand distribution p-value = 6.23e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
CTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGCCCGC > minE/2687094‑2687228
|
cTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAgg > 1:36607/1‑67 (MQ=255)
cTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGc > 1:365804/1‑68 (MQ=255)
cTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGc > 1:3306874/1‑68 (MQ=255)
cTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGc > 1:2713568/1‑68 (MQ=255)
cTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGc > 1:232029/1‑68 (MQ=255)
cTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGc > 1:1385561/1‑68 (MQ=255)
aGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGaa < 1:2293445/70‑1 (MQ=255)
aGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGaa < 1:2288659/70‑1 (MQ=255)
tACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAAcc < 1:549053/71‑1 (MQ=255)
tACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAAcc < 1:185585/71‑1 (MQ=255)
aCCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcg > 1:3498336/1‑71 (MQ=255)
aCCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcg > 1:3673676/1‑71 (MQ=255)
aGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCaa < 1:124997/71‑1 (MQ=255)
aGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCaa < 1:2987925/71‑1 (MQ=255)
aGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCaa < 1:3052028/71‑1 (MQ=255)
gTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAac < 1:116867/71‑1 (MQ=255)
gTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAac < 1:2154011/71‑1 (MQ=255)
gTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAac < 1:3083181/71‑1 (MQ=255)
ttGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAacac > 1:2625619/1‑71 (MQ=255)
tGGCGGTGAAGCGAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACg > 1:386554/1‑71 (MQ=255)
tGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACg > 1:3084067/1‑71 (MQ=255)
tGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACg > 1:1598359/1‑71 (MQ=255)
tGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACg > 1:3048923/1‑71 (MQ=255)
gCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAac < 1:2241583/66‑1 (MQ=255)
ggTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGa < 1:3964244/71‑1 (MQ=255)
ggTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGa < 1:1324371/71‑1 (MQ=255)
gTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGa < 1:1556651/70‑1 (MQ=255)
gTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGaa < 1:3898577/71‑1 (MQ=255)
gTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGaa < 1:2556903/71‑1 (MQ=255)
gAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAg > 1:1539793/1‑71 (MQ=255)
aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcgg > 1:2713765/1‑53 (MQ=255)
aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcgg > 1:1088133/1‑53 (MQ=255)
aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcgg > 1:2313494/1‑53 (MQ=255)
aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcgg > 1:1739259/1‑53 (MQ=255)
aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcgg > 1:1926969/1‑53 (MQ=255)
aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAAcc < 1:93029/51‑1 (MQ=255)
aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAAcc < 1:204673/51‑1 (MQ=255)
aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAAcc < 1:4113646/51‑1 (MQ=255)
aaGCCAGAGAGGAAGTGATACTCGGTTTGATCGGCAGTCAAGCGAGAAAcc < 1:1866825/51‑1 (MQ=255)
aGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCa > 1:2746050/1‑71 (MQ=255)
aGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCa > 1:3998399/1‑71 (MQ=255)
gCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCa > 1:964634/1‑70 (MQ=255)
gCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGaaa > 1:2822131/1‑47 (MQ=255)
gagagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcag > 1:2505281/1‑70 (MQ=255)
gagagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcag > 1:433317/1‑70 (MQ=255)
gagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgaga < 1:1706823/39‑1 (MQ=255)
gagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgaga < 1:2311391/39‑1 (MQ=255)
gagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgaga < 1:1323970/39‑1 (MQ=255)
gagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgaga < 1:1752432/39‑1 (MQ=255)
gagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCcaga < 1:3085877/39‑1 (MQ=255)
aGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcagc < 1:2054197/64‑1 (MQ=255)
aGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcagc < 1:2285906/64‑1 (MQ=255)
aGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcagc < 1:2276499/64‑1 (MQ=255)
aGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAg > 1:384883/1‑70 (MQ=255)
gTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAac < 1:2097657/46‑1 (MQ=255)
gTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAac < 1:2409918/46‑1 (MQ=255)
gTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAac < 1:3190513/46‑1 (MQ=255)
tGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAgg > 1:3962615/1‑69 (MQ=255)
tGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAgg > 1:299025/1‑69 (MQ=255)
tGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGaa < 1:1311052/71‑1 (MQ=255)
gATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAgg > 1:123239/1‑68 (MQ=255)
aTACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGa < 1:233922/71‑1 (MQ=255)
cTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAaca > 1:2929947/1‑41 (MQ=255)
cTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAaca > 1:2628215/1‑41 (MQ=255)
cTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAaca > 1:1296084/1‑41 (MQ=255)
tGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAAc > 1:576758/1‑71 (MQ=255)
gggTTTGATCGGCAGTCAAGCGAGAAACCGGAGGCAACACGCCGAAAGCATAAGCAGTCAGGAAGATAAcc > 1:2299513/1‑71 (MQ=255)
ggTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAAcc < 1:775589/70‑1 (MQ=255)
ggTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCt < 1:2502153/71‑1 (MQ=255)
gTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCTACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCtt < 1:3221188/71‑1 (MQ=255)
gTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAAcc < 1:2051769/69‑1 (MQ=255)
gTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAAcc < 1:191082/69‑1 (MQ=255)
tttGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCt > 1:798649/1‑69 (MQ=255)
tttGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTa < 1:1546122/71‑1 (MQ=255)
ttGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCtt > 1:2857125/1‑69 (MQ=255)
tGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGt > 1:2947200/1‑71 (MQ=255)
gCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGa < 1:965060/55‑1 (MQ=255)
tCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGaa < 1:2501467/49‑1 (MQ=255)
tCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGaa < 1:2100417/49‑1 (MQ=255)
tCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGaa < 1:1179141/49‑1 (MQ=255)
aGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAATATAACCTTAGTCGCGTGGCCCGc > 1:3276145/1‑71 (MQ=255)
aGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGCCCg > 1:1060305/1‑70 (MQ=255)
aGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGCCCGc > 1:3340338/1‑71 (MQ=255)
aGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGCCCGc > 1:1904152/1‑71 (MQ=255)
aGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGCCCGc > 1:1259279/1‑71 (MQ=255)
|
CTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGCCCGC > minE/2687094‑2687228
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
