Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R1
|
57 |
0.0 |
661283 |
90.4% |
597799 |
68.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
656,039 |
Δ1 bp |
100% |
coding (125/2601 nt) |
ycbS → |
predicted outer membrane usher protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 656,039 | 0 | C | . | 100.0%
| 16.7
/ NA
| 5 | coding (125/2601 nt) | ycbS | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (5/0); total (5/0) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCC > minE/656000‑656070
|
cAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTG‑TTTTTTCAAAGATGATCCCTCa > 1:482319/1‑61 (MQ=255)
cAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTG‑TTTTTTCAAAGATGATCCCTCAATGGTGGcc > 1:127500/1‑70 (MQ=255)
cAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTG‑TTTTTTCAAAGATGATCCCTCAATGGTGGcc > 1:189873/1‑70 (MQ=255)
cAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTG‑TTTTTTCAAAGATGATCCCTCAATGGTGGcc > 1:396754/1‑70 (MQ=255)
cAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTG‑TTTTTTCAAAGATGATCCCTCAATGGTGGcc > 1:582370/1‑70 (MQ=255)
|
CAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCC > minE/656000‑656070
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
