Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R1
|
57 |
0.0 |
661283 |
90.4% |
597799 |
68.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,302,407:1 |
+T |
100% |
coding (674/1167 nt) |
dacD ← |
D‑alanyl‑D‑alanine carboxypeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,302,407 | 1 | . | T | 100.0%
| 34.7
/ NA
| 13 | T225N (ACC→AAC) | dacD | D‑alanyl‑D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (0/13); total (0/13) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AACATTCATGG‑TTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTT > minE/1302397‑1302466
|
aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:103346/71‑1 (MQ=255)
aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:129425/71‑1 (MQ=255)
aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:357296/71‑1 (MQ=255)
aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:381785/71‑1 (MQ=255)
aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:420861/71‑1 (MQ=255)
aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:44034/71‑1 (MQ=255)
aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:520612/71‑1 (MQ=255)
aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:524366/71‑1 (MQ=255)
aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:551279/71‑1 (MQ=255)
aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:554080/71‑1 (MQ=255)
aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:603537/71‑1 (MQ=255)
aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:628173/71‑1 (MQ=255)
aCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:615713/70‑1 (MQ=255)
|
AACATTCATGG‑TTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTT > minE/1302397‑1302466
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
