Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R1
|
57 |
0.0 |
661283 |
90.4% |
597799 |
68.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,315,996:1 |
+T |
100% |
coding (594/738 nt) |
hisA → |
N‑(5'‑phospho‑L‑ribosyl‑formimino)‑5‑amino‑1‑ (5'‑phosphoribosyl)‑4‑imidazolecarboxamide isomerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,315,996 | 1 | . | T | 90.9%
| 32.7
/ ‑2.1
| 11 | coding (594/738 nt) | hisA | N‑(5'‑phospho‑L‑ribosyl‑formimino)‑5‑amino‑1‑ (5'‑phosphoribosyl)‑4‑imidazolecarboxamide isomerase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (10/0); total (11/0) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. |
AGGCTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCA‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCG > minE/1315942‑1316066
|
aGGCTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCA‑TTTCAGTCCTCcggcg > 1:401062/1‑71 (MQ=255)
tAACGTCTCTTTATATGAAGAAGTGTGTGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAt > 1:574210/1‑70 (MQ=255)
tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAtt > 1:319481/1‑71 (MQ=255)
tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAtt > 1:125975/1‑71 (MQ=255)
tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAtt > 1:395163/1‑71 (MQ=255)
tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAtt > 1:390433/1‑71 (MQ=255)
tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAtt > 1:627017/1‑71 (MQ=255)
tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAtt > 1:372090/1‑71 (MQ=255)
tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAtt > 1:370744/1‑71 (MQ=255)
tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAt > 1:372834/1‑70 (MQ=255)
tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGAGGCATTTTCAGTCCTCCGGCGGTAtt > 1:253659/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGTACTGGTGTGCgcggcg > 1:643664/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGgt > 1:188727/1‑61 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:449407/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:451814/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:574252/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:462263/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:474971/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:478491/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:441760/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:505788/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:517025/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:527534/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:538439/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:540514/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:427963/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:542022/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:426045/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:644760/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:106612/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:91179/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:87494/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:85157/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:82864/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:82730/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:75622/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:68256/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:653875/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:646615/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:555216/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:641558/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:637835/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:634550/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:611825/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:59485/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:5834/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:579731/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:97178/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:568786/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:238449/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:298848/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:425175/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:295624/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:295569/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:286513/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:285455/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:283357/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:259664/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:301944/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:212601/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:212092/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:184587/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:183570/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:181157/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:178965/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:169304/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:159604/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:348068/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:117810/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:398230/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:386090/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:362439/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:361982/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:357717/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:142911/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:351613/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:350668/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:347370/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:346755/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:344438/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:335953/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:335617/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:330110/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:308933/1‑71 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggc > 1:297875/1‑70 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggc > 1:158255/1‑70 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggc > 1:291876/1‑70 (MQ=255)
a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGAGGCGGCCCTGCGTGGCACTGGTGTGCgcggc > 1:406675/1‑70 (MQ=255)
a‑TTTCAGTCATCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:470238/1‑71 (MQ=255)
|
AGGCTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCA‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCG > minE/1315942‑1316066
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
