Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R1
|
57 |
0.0 |
661283 |
90.4% |
597799 |
68.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,398,903 |
T→C |
100% |
E576E (GAA→GAG) |
uvrD ← |
DNA‑dependent ATPase I and helicase II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,398,903 | 0 | T | C | 100.0%
| 13.5
/ NA
| 5 | E576E (GAA→GAG) | uvrD | DNA‑dependent ATPase I and helicase II |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (5/0); total (5/0) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TGGCTTGGGAACATGCCCTCTTCCATACCAACGATAAA > minE/2398883‑2398920
|
tGGCTTGGGAACATGCCCTCCTCCATACCAACGATaaa > 1:225632/1‑38 (MQ=255)
tGGCTTGGGAACATGCCCTCCTCCATACCAACGATaaa > 1:279292/1‑38 (MQ=255)
tGGCTTGGGAACATGCCCTCCTCCATACCAACGATaaa > 1:335220/1‑38 (MQ=255)
tGGCTTGGGAACATGCCCTCCTCCATACCAACGATaaa > 1:368397/1‑38 (MQ=255)
tGGCTTGGGAACATGCCCTCCTCCATACCAACGATaaa > 1:439037/1‑38 (MQ=255)
|
TGGCTTGGGAACATGCCCTCTTCCATACCAACGATAAA > minE/2398883‑2398920
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
