Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I3 R2
|
68 |
0.0 |
1607493 |
93.4% |
1501398 |
66.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,554,749 |
A→G |
D268D (GAT→GAC) |
adhP ← |
alcohol dehydrogenase, 1‑propanol preferring |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,554,749 | 0 | A | G | 96.7%
| 740.1
/ 7.7
| 209 | D268D (GAT→GAC) | adhP | alcohol dehydrogenase, 1‑propanol preferring |
| Reads supporting (aligned to +/- strand): ref base A (7/0); new base G (0/202); total (7/202) |
| Fisher's exact test for biased strand distribution p-value = 3.20e-13 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 |
Only 100 of 209 total aligned reads displayed.
GCGTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACAAGACGTGGGATATCCAGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAACAC > W3110S.gb/1554694‑1554792
|
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATTCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1070357/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:375933/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:585298/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:577383/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:555065/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:54744/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:505915/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:49856/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:485086/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:453026/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:434217/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:419123/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:393486/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:618146/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:35100/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:342726/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:331408/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:310278/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:300196/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:276404/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:264912/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:101763/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:248319/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:233411/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:821916/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:989847/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:971432/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:962071/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:954545/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:9446/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:921897/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:905013/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:883703/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:877618/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:863152/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:857558/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:604387/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:799172/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:737931/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:727025/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:713673/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:71186/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:701571/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:684089/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:664790/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:653256/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:253006/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1234141/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1381852/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1337195/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:133166/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:131323/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1300815/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1281498/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1279551/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1269625/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1263516/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1253319/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1247192/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1367856/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1223343/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1190698/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1162476/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1147007/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1122291/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1101110/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1083479/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1077065/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1024693/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1020691/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1511466/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:191729/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:185549/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:165176/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1603826/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1600992/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1591243/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1581079/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1573562/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1532052/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1524186/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1401430/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1496412/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1407217/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1474025/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1465593/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1437983/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1418751/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGACGTCCAGGCTCATAg < 1:149070/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGGGGGATGTCCAGGCTCATAg < 1:469926/69‑1 (MQ=255)
gcgTGCCGACCAGCGAACCAACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:408145/69‑1 (MQ=255)
cgTGCCGTCCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:134307/68‑1 (MQ=255)
cgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGTTGTCCAGGCTCATAg < 1:1211402/68‑1 (MQ=255)
cgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:381966/68‑1 (MQ=255)
cgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:563345/68‑1 (MQ=255)
cgTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:1055911/68‑1 (MQ=255)
gTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:763122/67‑1 (MQ=255)
ccAGCGAACCGACCACTTCAATACCATCCAGCACCAGCCGTGGGATGTCCAGGCTCATAg < 1:312583/60‑1 (MQ=255)
tACCATCCAGCACAAGACGTGGGATATCCAGGCTCATAGACTCcggcgg > 1:589859/1‑49 (MQ=255)
tACCATACAGCACAAGACGTGGGATATCCAGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAacac > 1:216/1‑69 (MQ=255)
|
GCGTGCCGACCAGCGAACCGACCACTTCAATACCATCCAGCACAAGACGTGGGATATCCAGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAACAC > W3110S.gb/1554694‑1554792
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
