Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I3 R2 68 0.0 1607493 93.4% 1501398 66.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,566,303 T→C C8C (TGT→TGC)  yihR → predicted aldose‑1‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,566,3030TC100.0% 510.4 / NA 136C8C (TGT→TGC) yihRpredicted aldose‑1‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/136);  total (0/136)

Only 100 of 136 total aligned reads displayed.

TAAGGTGCCTTGTATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCGGGGG  >  W3110S.gb/3566291‑3566358
            |                                                       
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:507536/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:733251/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:71747/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:716239/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:682718/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:641105/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:628251/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:626530/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:617784/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:578679/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:577458/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:5516/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:53649/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:744078/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:472978/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:470000/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:448359/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:436107/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:426925/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:362803/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:338952/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:307604/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:300575/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:296174/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:287670/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:81440/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:970854/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:940020/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:938765/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:916484/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:913218/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:911470/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:878218/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:868537/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:858366/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:842290/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:826463/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1008606/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:811890/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:809757/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:794658/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:791182/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:782582/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:779269/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:7745/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:770373/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:764000/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:759201/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:74996/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1149349/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1329478/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1312632/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1297760/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1284922/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1263789/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:122133/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1215022/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1213526/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1197990/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1197820/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1171336/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:264768/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1146774/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1137409/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1127604/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1125741/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1097114/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1092286/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1083349/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1060854/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1057281/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1025785/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1002207/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1372751/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:277734/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:221693/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:221103/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:210955/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:198387/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:178244/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1565924/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1556440/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1545636/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1541998/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:152175/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1470746/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1462109/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1459964/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:145203/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1436591/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1405454/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1403407/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1399766/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1396897/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1373292/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1369296/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCCCggggg  <  1:205567/68‑1 (MQ=255)
taaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGAGGACAGACCGTTTCACTTGCCGCggggg  <  1:991755/68‑1 (MQ=255)
aaaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:940508/67‑1 (MQ=255)
 aaGGTGCCTTGCATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCggggg  <  1:1056454/67‑1 (MQ=255)
            |                                                       
TAAGGTGCCTTGTATGCAAATCACCAATATGCATTGCAGTGGACAGACCGTTTCACTTGCCGCGGGGG  >  W3110S.gb/3566291‑3566358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A