Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F17 I0 R1	71	95.7	2922988	91.7%	2680379	65.6

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,602,012:1	+A	100%	coding (853/1305 nt)	yfeW →	predicted periplasmic esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,602,012	1	.	A	84.6%	29.6 / 1.9	13	coding (853/1305 nt)	yfeW	predicted periplasmic esterase
Reads supporting (aligned to +/- strand): ref base . (2/0); new base A (11/0); total (13/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

ATCCATTTCCCGAATATCCGCACCTCCACTCTCTGGGGTCAGGTGCACGATG‑AAAAAGCCTTTTATTCGATGGGCGGCGTTTCCGGG  >  minE/1601961‑1602047

aTCCATTTCCCGAATATCCGCACCTCCACTCTCTGGGGTCAGGTGCACGATG‑AAAAAGCCTTTTATTCGa                   >  1:640958/1‑70 (MQ=255)

 tCCATTTCCCGAATATCCGCACCTCCACTCTCTGGGGTCAGGTGCACGATG‑AAAAAGCCTTTTATTCGATg                 >  1:1746134/1‑71 (MQ=255)

                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:1546306/1‑70 (MQ=255)

                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:1644358/1‑70 (MQ=255)

                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:1674577/1‑70 (MQ=255)

                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:2656268/1‑70 (MQ=255)

                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:2780840/1‑70 (MQ=255)

                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:317654/1‑70 (MQ=255)

                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:479529/1‑70 (MQ=255)

                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:49296/1‑70 (MQ=255)

                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:642915/1‑70 (MQ=255)

                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:871978/1‑70 (MQ=255)

                  cGCACCTACACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:2456977/1‑70 (MQ=255)

ATCCATTTCCCGAATATCCGCACCTCCACTCTCTGGGGTCAGGTGCACGATG‑AAAAAGCCTTTTATTCGATGGGCGGCGTTTCCGGG  >  minE/1601961‑1602047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A