Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R1
|
71 |
95.7 |
2922988 |
91.7% |
2680379 |
65.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,947,335 |
A→G |
100% |
L158P (CTG→CCG) |
dnaT ← |
DNA biosynthesis protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,947,335 | 0 | A | G | 100.0%
| 169.2
/ NA
| 45 | L158P (CTG→CCG) | dnaT | DNA biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (45/0); total (45/0) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GTTCGCTGACCGTATTCACATCTCGTTTCGGCAGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTGCGCG > minE/2947303‑2947374
|
gTTCGCTGACCGTATTCACATCTCGTTTGGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:314763/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTcc > 1:962479/1‑37 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTg > 1:1800786/1‑61 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:1066518/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:528477/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:495542/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:471888/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:2855413/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:2617461/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:2481103/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:2381762/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:2241625/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:2079976/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:1465009/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:1136016/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:1097226/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:1433708/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:1294107/1‑71 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcg > 1:103340/1‑70 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcg > 1:2296617/1‑70 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcg > 1:1782397/1‑70 (MQ=255)
gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcg > 1:177134/1‑70 (MQ=255)
ttagCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:1155022/4‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGt > 1:1828377/1‑41 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:640573/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:609567/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:1108418/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:1130422/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:672370/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:2904685/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:128046/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:2770665/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:2659974/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:2549858/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:1226274/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:2379289/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:2311088/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:2257511/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:1285418/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:2176553/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:2139818/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:212807/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:156082/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg > 1:1536391/1‑71 (MQ=255)
ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc > 1:1201487/1‑70 (MQ=255)
|
GTTCGCTGACCGTATTCACATCTCGTTTCGGCAGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTGCGCG > minE/2947303‑2947374
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
