Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F17 I0 R2
|
276 |
45.3 |
3729697 |
93.0% |
3468618 |
60.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
488,522 |
A→T |
23.2% |
V921V (GTA→GTT) |
kefA → |
fused mechanosensitive channel proteins |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 488,522 | 0 | A | T | 23.2%
| 131.9
/ 42.6
| 69 | V921V (GTA→GTT) | kefA | fused mechanosensitive channel proteins |
| Reads supporting (aligned to +/- strand): ref base A (25/28); new base T (12/4); total (37/32) |
| Fisher's exact test for biased strand distribution p-value = 8.46e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.30e-01 |
ACGGTGTTCGGATCGCTGGGCGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTAT > W3110S.gb/488457‑488583
|
aCGGTGTTCGGATCGCTGGGCGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGt > 1:1651090/1‑69 (MQ=255)
gtgtTCGGATCGCTGGGCGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCtt < 1:1410315/69‑1 (MQ=255)
ttCGGATCGCTGGGCGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCttggt > 1:865522/1‑69 (MQ=255)
tCGCTGGGCGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCttggttttgg > 1:3279887/1‑68 (MQ=255)
cGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGa > 1:1987701/1‑69 (MQ=255)
cGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGa < 1:2972838/69‑1 (MQ=255)
cGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGa < 1:1930894/69‑1 (MQ=255)
gggATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTc > 1:2571023/1‑68 (MQ=255)
gggATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTc > 1:1421931/1‑68 (MQ=255)
gATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt > 1:3327213/1‑69 (MQ=255)
gATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt > 1:2362886/1‑69 (MQ=255)
gATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt > 1:1220671/1‑69 (MQ=255)
gATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt > 1:1428916/1‑69 (MQ=255)
aaaCTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt < 1:169969/66‑1 (MQ=255)
aaaCTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt < 1:1962653/66‑1 (MQ=255)
aaCTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACt > 1:3358038/1‑69 (MQ=255)
aCTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGaaa < 1:2826014/56‑1 (MQ=255)
cTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAAtt < 1:79218/57‑1 (MQ=255)
cTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAAtt < 1:1242613/57‑1 (MQ=255)
cTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAAtt < 1:2573322/57‑1 (MQ=255)
cTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAAtt < 1:1003560/57‑1 (MQ=255)
cTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCTGTAACTTc < 1:2820154/69‑1 (MQ=255)
cTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt < 1:2535051/63‑1 (MQ=255)
cTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTc < 1:981728/69‑1 (MQ=255)
cAGTGGCTGGCCGCAGCATTATCCGTAGGTCttggt > 1:3292166/1‑36 (MQ=255)
cAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGtc > 1:1829842/1‑69 (MQ=255)
tggctggcCGCAGCATTATCCGTAGGTCttggttttggt < 1:1090845/39‑1 (MQ=255)
tggctggcCGCAGCATTATCCGTAGGTCttggttttggt < 1:2862261/39‑1 (MQ=255)
tggctggcCGCAGCATTATCCGTAGGTCttggttttggt < 1:2232771/39‑1 (MQ=255)
ggctggcCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCg > 1:1925575/1‑69 (MQ=255)
cGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTg < 1:1956525/67‑1 (MQ=255)
cGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTg < 1:1323852/67‑1 (MQ=255)
cGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGAt < 1:927947/69‑1 (MQ=255)
cGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGAt < 1:848707/69‑1 (MQ=255)
cGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGAt < 1:442615/69‑1 (MQ=255)
gcagcaTTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGAt < 1:2899112/68‑1 (MQ=255)
caTTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTc < 1:228123/69‑1 (MQ=255)
caTTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTc < 1:1917903/69‑1 (MQ=255)
caTTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTc < 1:2024464/69‑1 (MQ=255)
caTTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTc < 1:2806199/69‑1 (MQ=255)
caTTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTc < 1:1249509/69‑1 (MQ=255)
gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:797325‑M1/3‑26 (MQ=255)
gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:870414‑M1/3‑26 (MQ=255)
gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:379962‑M1/3‑26 (MQ=255)
gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:897869‑M1/3‑26 (MQ=255)
gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:1171766‑M1/3‑26 (MQ=255)
gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg < 1:3242849‑M1/40‑17 (MQ=255)
gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:2988575‑M1/3‑26 (MQ=255)
gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:2450555‑M1/3‑26 (MQ=255)
gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:3060973‑M1/3‑26 (MQ=255)
aTTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTc < 1:95966/68‑1 (MQ=255)
ttATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCt > 1:1848944/1‑68 (MQ=255)
ttATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTa > 1:2641476/1‑69 (MQ=255)
tATCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttggt > 1:1678224‑M1/1‑24 (MQ=255)
tATCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttggt > 1:927043‑M1/1‑24 (MQ=255)
tATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTa > 1:2015671/1‑68 (MQ=255)
aTCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttggt < 1:3281144‑M1/40‑18 (MQ=255)
aTCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt > 1:840139/1‑40 (MQ=255)
aTCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTa > 1:2258341/1‑67 (MQ=255)
aTCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTAt > 1:2853629/1‑68 (MQ=255)
aTCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTAt > 1:2328173/1‑68 (MQ=255)
aTCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTAt > 1:2176695/1‑68 (MQ=255)
aTCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTAt > 1:2597303/1‑68 (MQ=255)
aTCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTAt > 1:3046144/1‑68 (MQ=255)
tCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:443368‑M1/1‑22 (MQ=255)
tCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:430822‑M1/1‑22 (MQ=255)
tCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTc > 1:1964438/1‑64 (MQ=255)
ccGTTGGTTTTGGTTTCGGTTgtgaaaagtttttt < 1:3031426‑M1/35‑15 (MQ=255)
cGTTGGTTTTGGTTTCGGTTgtgaaaagttttttggt < 1:2979144‑M1/37‑18 (MQ=255)
|
ACGGTGTTCGGATCGCTGGGCGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTAT > W3110S.gb/488457‑488583
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
