Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
249,667 |
C→A |
37.7% |
A71A (GCC→GCA) |
proY → |
predicted cryptic proline transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 249,667 | 0 | C | A | 37.7%
| 43.5
/ 40.0
| 53 | A71A (GCC→GCA) | proY | predicted cryptic proline transporter |
| Reads supporting (aligned to +/- strand): ref base C (19/14); new base A (11/9); total (30/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 |
TTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGG > minE/249600‑249733
|
ttatCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGgcc > 1:198385/1‑68 (MQ=255)
ttatCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGgcc > 1:1881622/1‑68 (MQ=255)
atCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCTgccgc > 1:1075535/1‑68 (MQ=255)
atCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGgccgcc > 1:2810986/1‑69 (MQ=255)
atCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGgccgcc > 1:227746/1‑69 (MQ=255)
atCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGgccgcc > 1:2159362/1‑69 (MQ=255)
aTCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTtc > 1:885318/1‑69 (MQ=255)
gcgTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGt < 1:153145/69‑1 (MQ=255)
tatCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATg > 1:1657285/1‑68 (MQ=255)
tCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATgcgc > 1:2185282/1‑69 (MQ=255)
tgcgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATgcgc > 1:1025047/1‑63 (MQ=255)
tgcgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATgcgc > 1:1000671/1‑63 (MQ=255)
tgcgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATgcgc > 1:1182171/1‑63 (MQ=255)
cgtgcgCTGGGGGATATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAAc < 1:3048534/69‑1 (MQ=255)
cgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAAc < 1:3175740/69‑1 (MQ=255)
cgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAAc < 1:2200015/69‑1 (MQ=255)
cgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAAc < 1:1211424/69‑1 (MQ=255)
cgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAAc < 1:2455172/69‑1 (MQ=255)
cgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAAc < 1:1337439/69‑1 (MQ=255)
tCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGt > 1:3132930/1‑69 (MQ=255)
tCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGt > 1:2277902/1‑69 (MQ=255)
tCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGt > 1:4828/1‑69 (MQ=255)
tCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTAGGCCCGCTGGCAGGt > 1:1079337/1‑69 (MQ=255)
ggTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGGAGGAAAAc < 1:377336/49‑1 (MQ=255)
ggTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTa < 1:375142/69‑1 (MQ=255)
ggTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTa < 1:3129976/69‑1 (MQ=255)
gTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTa < 1:608713/68‑1 (MQ=255)
cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTTGCAGGTTACAtt > 1:3147312/1‑69 (MQ=255)
cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:3147564/1‑69 (MQ=255)
cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:3048662/1‑69 (MQ=255)
cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:2132694/1‑69 (MQ=255)
cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:2090214/1‑69 (MQ=255)
cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:413541/1‑69 (MQ=255)
cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:1980048/1‑69 (MQ=255)
cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:1800412/1‑69 (MQ=255)
cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:1763325/1‑69 (MQ=255)
cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:1739943/1‑69 (MQ=255)
cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTAGGCCCGCTGGCAGGTTACAtt > 1:2665960/1‑69 (MQ=255)
atcaaCCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt < 1:448888/66‑1 (MQ=255)
atcaaCCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt < 1:2653142/66‑1 (MQ=255)
atcaaCCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt < 1:526038/66‑1 (MQ=255)
tAACCCGGCCTCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAc > 1:2681408/1‑69 (MQ=255)
tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCgg > 1:2170786/1‑48 (MQ=255)
tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAt > 1:1607091/1‑66 (MQ=255)
tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAc > 1:383064/1‑69 (MQ=255)
tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAc > 1:2638407/1‑69 (MQ=255)
tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAc > 1:1060625/1‑69 (MQ=255)
tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAc > 1:2290000/1‑69 (MQ=255)
tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAc > 1:2112862/1‑69 (MQ=255)
tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAc > 1:1441672/1‑69 (MQ=255)
ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGTCCCGCTGGCAGGTTACATTACCGGc < 1:289958/69‑1 (MQ=255)
ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:2914348/69‑1 (MQ=255)
ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:2761734/69‑1 (MQ=255)
ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:1467317/69‑1 (MQ=255)
ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:2216204/69‑1 (MQ=255)
ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:1240917/69‑1 (MQ=255)
ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:1926456/69‑1 (MQ=255)
ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:1343827/69‑1 (MQ=255)
ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:1642640/69‑1 (MQ=255)
gccgccAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTgg < 1:647320/69‑1 (MQ=255)
gccgccAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTgg < 1:1528157/69‑1 (MQ=255)
|
TTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGG > minE/249600‑249733
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
