Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
271,058 |
C→T |
22.2% |
I347I (ATC→ATT) |
thiI → |
sulfurtransferase required for thiamine and 4‑thiouridine biosynthesis |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 271,058 | 0 | C | T | 22.2%
| 90.9
/ 18.0
| 67 | I347I (ATC→ATT) | thiI | sulfurtransferase required for thiamine and 4‑thiouridine biosynthesis |
| Reads supporting (aligned to +/- strand): ref base C (32/20); new base T (6/9); total (38/29) |
| Fisher's exact test for biased strand distribution p-value = 1.54e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 |
ATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAG > minE/270990‑271126
|
atcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGAtc > 1:601046/1‑69 (MQ=255)
atcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGAtc > 1:1725845/1‑69 (MQ=255)
atcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGAtc > 1:273537/1‑69 (MQ=255)
atcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGAtc > 1:1575951/1‑69 (MQ=255)
aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGaaaacg < 1:2207785/69‑4 (MQ=255)
aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:1871534/69‑2 (MQ=255)
aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:1972464/69‑2 (MQ=255)
aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:173271/69‑2 (MQ=255)
aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:2041604/69‑2 (MQ=255)
aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:2097563/69‑2 (MQ=255)
aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:1573960/69‑2 (MQ=255)
aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:2973753/69‑2 (MQ=255)
aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:586771/69‑2 (MQ=255)
gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:2819559/1‑68 (MQ=255)
gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:14534/1‑68 (MQ=255)
gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:1421980/1‑68 (MQ=255)
gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:2209153/1‑68 (MQ=255)
gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:3134418/1‑68 (MQ=255)
gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:3372047/1‑68 (MQ=255)
gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:538427/1‑68 (MQ=255)
gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:1153071/1‑68 (MQ=255)
gAAGACTTTGCTCGCACGATGCCGGAATATTGAGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:3069275/1‑68 (MQ=255)
aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:3076905/69‑1 (MQ=255)
aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:2097588/69‑1 (MQ=255)
aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:2067669/69‑1 (MQ=255)
aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:997718/69‑1 (MQ=255)
aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:1423431/69‑1 (MQ=255)
aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:1178106/69‑1 (MQ=255)
gACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGAc < 1:2220979/53‑1 (MQ=255)
gACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGAc < 1:63537/53‑1 (MQ=255)
gACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGAc < 1:3296063/53‑1 (MQ=255)
gACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:2231866/68‑1 (MQ=255)
cTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGaa < 1:1245701/67‑1 (MQ=255)
gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGaa > 1:1334117/1‑49 (MQ=255)
gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGAt > 1:875763/1‑67 (MQ=255)
gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGAt > 1:1968003/1‑67 (MQ=255)
gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGAt > 1:2504950/1‑67 (MQ=255)
gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGAt > 1:1615125/1‑67 (MQ=255)
gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGAt > 1:1607771/1‑67 (MQ=255)
ggAATATTGTGGTGTTATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:1232000/68‑1 (MQ=255)
ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:2613056/68‑1 (MQ=255)
ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:1872466/68‑1 (MQ=255)
ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:3043280/68‑1 (MQ=255)
ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:1404595/68‑1 (MQ=255)
ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:364317/68‑1 (MQ=255)
ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:1654374/68‑1 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:3385502/1‑69 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1332081/1‑69 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1218746/1‑69 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:756310/1‑69 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:303766/1‑69 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:7852/1‑69 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1049347/1‑69 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:3025362/1‑69 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:2976152/1‑69 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1924980/1‑69 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:228465/1‑69 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1703369/1‑69 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:2139018/1‑69 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:2064556/1‑69 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagaga < 1:2572847/68‑1 (MQ=255)
ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagaga < 1:1650191/68‑1 (MQ=255)
ttGTGGTGTGATCTACAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1927161/1‑69 (MQ=255)
gtgtGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCg > 1:736546/1‑69 (MQ=255)
gtgtGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCg > 1:1104579/1‑69 (MQ=255)
tgATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGGTTGAAGCGGAAGAAGAGAAGTTCGAc > 1:3200732/1‑68 (MQ=255)
tgATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGAc > 1:1588151/1‑68 (MQ=255)
tgATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGAc > 1:1863472/1‑68 (MQ=255)
tgATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGAc > 1:2640725/1‑68 (MQ=255)
aTCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCg > 1:2213489/1‑64 (MQ=255)
aTCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCg > 1:3193667/1‑64 (MQ=255)
ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagaga > 1:465156/1‑56 (MQ=255)
ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagaga > 1:1599938/1‑56 (MQ=255)
ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagaga > 1:2662407/1‑56 (MQ=255)
ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAg > 1:484520/1‑69 (MQ=255)
ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAg > 1:1998754/1‑69 (MQ=255)
ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAg > 1:79105/1‑69 (MQ=255)
ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAg > 1:929800/1‑69 (MQ=255)
ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAA‑AGAAGTTCGACTTCAg < 1:2072295/68‑1 (MQ=255)
|
ATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAG > minE/270990‑271126
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
